Mouse anti Lamin A, conjugated to FITC, Primary Antibodies
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Catalog number
MUB1101L1
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Price
Please ask
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Size
1ml
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Host Source
Mouse
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Clone Name
133A2
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Product Category
Cytoskeleton, Neuroscience, Cell Biology
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Species Reactivity
Cattle, Dog, Human, Mouse, Rat
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Application
Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen)
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Immunogen
133A2 is a mouse monoclonal IgG3/_ antibody obtained from fusion of P3/X63.Ag8.653 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with partially purified recombinant human lamin A.
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Purification Method
NA
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Shipping Conditions
NA
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Storage conditions
Mouse anti Lamin A, conjugated to FITC, Primary Antibodies t 4oC, or in small aliquots at -20oC.
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Datasheet link
NA
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Properties
This Exalpha Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Exalpha FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Gene target
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Short name
Mouse anti Lamin A, conjugated FITC, Primary Antibodies
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Technique
Mouse, anti, FITC, antibody to, antibody Conjugates, Fluorescein, mouses
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Host
mouse
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Isotype
IgG3
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Label
FITC
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Species
Mouse, Mouses
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Alternative name
Mouse antibody to Lamin A, coupled to fluorecein, captor Antibodies
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Alternative technique
murine, antibodies, fluorescine
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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