-
Host Source
Goat
-
Clone Name
Polyclonal
-
Product Category
Veterinary
-
Species Reactivity
Rat
-
Application
ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence
-
Immunogen
Purified polyclonal IgG and homogenous IgA and IgM isolated from rat serum. FreundÍs complete adjuvant is used in the first step of the immunization procedure.
-
Purification Method
NA
-
Shipping Conditions
NA
-
Storage conditions
Goat anti Rat IgG1 IgG2a IgG2b IgG2c IgA IgM (Fc specific), conjugated with FITC, Secondary Antibodies philized conjugate is shipped at ambient temperature and may be stored at +4ÁC; prolonged storage at or below -20ÁC. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20ÁC. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4ÁC, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate. Lyophilized at +4Á C--at least 10 years. Reconstituted at or below -20Á C--3-5 years. Reconstituted at +4Á C--7 days
-
Datasheet link
NA
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
-
Properties
This Exalpha Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Exalpha FITC is highly sensitive to pH extremes. Immunoglobulin A (IgA, also referred to as sIgA) is an antibody that plays a critical role in immune function in the mucous membranes. More IgA is produced in mucosal linings than all other types of antibody combined. Examples are EMA, ACA ELISA test kits and for diagnostic detection the IgA of Anti-Beta-2 Glycoprotein 1; β2-Glycoprotein 1 Antibodies; Beta 2GP1 Ab, Beta-2 Glycoprotein 1 Antibodies IgG, IgM, and IgAs. Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen
-
Conjugation
Anti-FITC Antibody
-
Latin name
Capra aegagrus hircus, Rattus norvegicus
-
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
-
Gene target
-
Short name
Goat anti IgG1 IgG2a IgG2b IgG2c IgA IgM (Fc specific), conjugated with FITC, Secondary Antibodies
-
Technique
anti, FITC, Goat, IgA, IgM, FC, antibody to, antibody Conjugates, Fluorescein, goats, IgAs, IgMs
-
Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
-
Isotype
NA, IgA, IgG1, IgG2a, IgG2b, IgG2c, IgM
-
Label
FITC
-
Species
Rat, Goats, Rats
-
Alternative name
caprine antibody to Rat IgG1 IgG2a IgG2b IgG2c Immunoglobulin A Immunoglobulin M (fragment c specific), coupled including fluorecein, secondary detector Antibody Antibodies
-
Alternative technique
antibodies, caprine, fluorescine, immunoglobulin-a, immunoglobulin-m
-