Goat anti Mouse IgG1 IgG2a IgG2b IgG3 IgA IgM (heavy and light chains), conjugated with FITC, Secondary Antibodies
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Catalog numberGAM-Ig-FITC
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Price:
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Size1ml
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Host SourceGoat
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Clone NamePolyclonal
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Product CategoryVeterinary
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Species ReactivityMouse
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ApplicationELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence
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ImmunogenPurified polyclonal mouse IgG, and homogenous IgA and IgM isolated from mouse serum. FreundÍs complete adjuvant is used in the first step of the immunization procedure.
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Purification MethodNA
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Shipping ConditionsNA
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Storage conditionsGoat anti Mouse IgG1 IgG2a IgG2b IgG3 IgA IgM (heavy and light chains), conjugated with FITC, Secondary Antibodies philized conjugate is shipped at ambient temperature and may be stored at +4ÁC; prolonged storage at or below -20ÁC. It is reconstituted by adding 1 ml sterile distilled water. Spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20ÁC. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4ÁC, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate. Lyophilized at +4Á C--at least 10 years. Reconstituted at or below -20Á C--3-5 years. Reconstituted at +4Á C--7 days
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Datasheet linkNA
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. This is against a protein of mus musculus directed antibody.
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PropertiesThis Exalpha Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Exalpha FITC is highly sensitive to pH extremes. Immunoglobulin A (IgA, also referred to as sIgA) is an antibody that plays a critical role in immune function in the mucous membranes. More IgA is produced in mucosal linings than all other types of antibody combined. Examples are EMA, ACA ELISA test kits and for diagnostic detection the IgA of Anti-Beta-2 Glycoprotein 1; β2-Glycoprotein 1 Antibodies; Beta 2GP1 Ab, Beta-2 Glycoprotein 1 Antibodies IgG, IgM, and IgAs. Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen
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ConjugationAnti-FITC Antibody
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Latin nameCapra aegagrus hircus, Mus musculus
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Gene target
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Short nameGoat anti Mouse IgG1 IgG2a IgG2b IgG3 IgA IgM (heavy light chains), conjugated with FITC, Secondary Antibodies
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Techniqueanti mouse, light, Mouse, anti, FITC, Goat, IgA, IgM, antibody to, anti mouse antibodies, antibody Conjugates, Fluorescein, goats, IgAs, IgMs, mouses
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HostGoat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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IsotypeNA, IgA, IgG1, IgG2a, IgG2b, IgG3, IgM
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LabelFITC
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SpeciesMouse, Goats, Mouses
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Alternative namecaprine antibody to Mouse IgG1 IgG2a IgG2b IgG3 Immunoglobulin A Immunoglobulin M (heavy and light chains), coupled including fluorecein, secondary detector Antibody Antibodies
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Alternative techniquemurine, antibodies, caprine, fluorescine, immunoglobulin-a, immunoglobulin-m
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data