CD8 FITC - HLADR PE, Bi-Test Reagents (FITC/RPE)
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Catalog number
08DR
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Price
Please ask
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Size
100Tests
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Host Source
Mouse
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Clone Name
NA
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Product Category
Flow Cytometry
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Species Reactivity
Human
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Application
Flow Cytometry
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Immunogen
CD8=Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human perherial blood T lymphocytes.
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Purification Method
Protein A/G Chromatography
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Shipping Conditions
Room Temperature
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Storage conditions
CD8 FITC - HLADR PE, Bi-Test Reagents (FITC/RPE) should be stored at 4-8ÂșC. DO NOT FREEZE
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Description
Cytotoxic T cells cluster differentiators for flow cytometry. This transmembrane glycoprotein is detected by monoclonals and has a lot of clones available with different affinity.
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Properties
This Exalpha Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Exalpha FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Gene target
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Gene symbol
RPE, CD8A
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Short name
CD8 FITC - HLADR PE, Bi-Test Reagents (FITC/RPE)
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Technique
FITC, Fluorescein
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Isotype
NA
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Label
FITC
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Alternative name
CD8 fluorecein - HLADR peroxidase, Bi-Test Reagents (fluorecein/RPE)
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Alternative technique
tests, fluorescine
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Gene info
Gene info
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Identity
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Gene
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Long gene name
CD8a molecule
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Synonyms gene
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Synonyms gene name
- CD8 antigen, alpha polypeptide (p32)
- T-cell surface glycoprotein CD8 alpha chain
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Locus
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Discovery year
1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- V-set domain containing
- CD molecules
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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Concept
Scope note:
Testing erythrocytes to determine presence or absence of blood-group antigens, testing of serum to determine the presence or absence of antibodies to these antigens, and selecting biocompatible blood by crossmatching samples from the donor against samples from the recipient. Crossmatching is performed prior to transfusion.
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Tree numbers
- E01.370.225.625.120
- E01.370.225.812.385.120
- E05.200.625.120
- E05.200.812.385.120
- E05.478.594.385.120
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Qualifiers
ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data
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