TNF Polyclonal Antibody, Biotin Conjugated

Note

For research use only.
Properties

If you buy Antibodies supplied by EpiGentek they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation

Biotinylated
Group

Polyclonals and antibodies
About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
Gene

Tumor necrosis factor (TNFa, tumor necrosis factor alpha, TNFα, cachexin, or cachectin) is a cell signaling protein (cytokine) involved in systemic inflammation and is one of the cytokines that make up the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. TNFb or TNF beta also bin on TNF receptors for Th1 activation.
French translation

anticorps

Gene target

TNF Conjugated
Gene symbol

TNF, TNFRSF1A, TNFRSF1B, LTBR
Short name

TNF Polyclonal Antibody, Conjugated
Technique

Polyclonal, Antibody, antibodies against human proteins, antibodies for, antibody Conjugates, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
Label

Biotin
Alternative name

tumor necrosis factor polyclonal (antibody to-), biotinilated coupled
Alternative technique

polyclonals, antibodies
Alternative to gene target

tumor necrosis factor, DIF and TNF-alpha and TNFA and TNFSF2, TNF and IDBG-300259 and ENSG00000232810 and 7124, transcription regulatory region DNA binding, Extracellular, Tnf and IDBG-177358 and ENSMUSG00000024401 and 21926, TNF and IDBG-634161 and ENSBTAG00000025471 and 280943

Identity

HGNC:11916
Gene

TNFRSF1A
Long gene name

TNF receptor superfamily member 1A
Synonyms gene
- TNFR1
Synonyms gene name
- tumor necrosis factor receptor superfamily, member 1A
Synonyms
- TNF-R
- TNFAR
- TNFR60
- TNF-R-I
- CD120a
- TNF-R55
GenBank acession
- M75866
Locus

12p13.31
Discovery year

1991-01-15
Entrez gene record

7132
Pubmed identfication
- 1655358
- 2158863
RefSeq identity
- NM_001065
Classification
- Tumor necrosis factor receptor superfamily
- CD molecules
VEGA ID

OTTHUMG00000168267
Locus Specific Databases
- INFEVERS: The repertory of Familial Mediterranean Fever (FMF) and Hereditary Inflammatory Disorders Mutations
- LRG_193

Identity

HGNC:11917
Gene

TNFRSF1B
Long gene name

TNF receptor superfamily member 1B
Synonyms gene
- TNFR2
Synonyms gene name
- tumor necrosis factor receptor superfamily, member 1B
Synonyms
- TNFBR
- TNFR80
- TNF-R75
- TNF-R-II
- p75
- CD120b
GenBank acession
- M32315
Locus

1p36.22
Discovery year

1991-01-15
Entrez gene record

7133
Pubmed identfication
- 2158863
- 8702885
RefSeq identity
- NM_001066
Classification
- CD molecules
- MicroRNA protein coding host genes
- Tumor necrosis factor receptor superfamily
VEGA ID

OTTHUMG00000001829

Name

Blotting, Western
Concept

Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data