Rat Serum Albumin Polyclonal Antibody, FITC Conjugated
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Catalog number
A57523-100
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Price
Please ask
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Size
100 µg
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Storage
Our specialists from Gentaur/Genprice advise to follow carefully the instructions for use and store the Rat Serum Albumin Polyclonal Antibody, FITC Conjugated in refrigerator and according to the label upon arrival.
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Tips
Manufactured in United States. Designed for Research Use Only, not for human or animal consumption.
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Properties
If you buy Antibodies supplied by Epigen they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Epigen Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Epigen FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Group
Polyclonals and antibodies, Sera
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats. Serum should be stored at +5°C
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Latin name
Rattus norvegicus
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Description
Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.
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French translation
anticorps
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Gene target
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Short name
Serum Albumin Polyclonal Antibody, FITC Conjugated
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Technique
Polyclonal, Antibody, serum, FITC, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rat
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Label
FITC
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Species
Rat, Rats
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Alternative name
Rat blood serum Albumin polyclonal (antibody to-), fluorecein coupled
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Alternative technique
polyclonals, antibodies, sera, fluorescine
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Tissue
serum
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MeSH Data
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Name
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Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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