U-2 OS [U2OS] Cell Line
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Catalog numberEP-CL-0236
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Price:
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Size1×10⁶ cells/vial
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AbbreviationU-2 OS [U2OS]
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Leadtime3-5 days
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Shipping conditionDry ice
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StorageLiquid nitrogen -196°C
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AgeNA
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MorphologyEpithelial
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Growth_propertiesAdherent
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Doubling_timeNA
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BackgroundNA
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Biosafety1
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MediumMcCoy's 5A (PM150710)+10% FBS (164210-500)+1% P/S (PB180120)
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SubculturingRemove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
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Ratio1:2-1:4
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RenewalEvery 2 to 3 days
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CryopreservationFreeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
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Culture_conditionsAtmosphere: Air, 95%; CO2, 5% Temperature: 37℃
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TumorigenicNA
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EffectsNA
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Receptor_expressionNA
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Antigen_expressionNA
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Gene_expressionNA
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ApplicationsNA
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DurationNA
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DescriptionFor cells, cell lines and tissues in culture till half confluency.
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Gene target
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Gene symbolRNU2-2P, RNU2-4P, RNU2-3P, RNU2-1, HLA-U, UTS2, RNU2-5P, CENPU, PTPRO, MIR1289-2
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Short nameU-2 OS [U2OS] Line
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SpeciesHomo sapiens, Human
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Alternative nameU-2 OS [U2OS] cellular Line
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TissueBone
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Gene info
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Identity
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Gene
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Long gene nameRNA, U2 small nuclear 2, pseudogene
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Synonyms gene
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Synonyms gene name
- RNA, U2B small nuclear
- RNA, U2 small nuclear 2
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-12-14
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Entrez gene record
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Pubmed identfication
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RefSeq identity
Gene info
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Identity
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Gene
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Long gene nameRNA, U2 small nuclear 4, pseudogene
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Synonyms gene
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Synonyms gene name
- RNA, U2 small nuclear pseudogene 2
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-12-14
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Entrez gene record
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Pubmed identfication
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RefSeq identity
Gene info
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Identity
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Gene
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Long gene nameRNA, U2 small nuclear 3, pseudogene
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Synonyms gene
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Synonyms gene name
- RNA, U2 small nuclear pseudogene 1
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-12-14
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Entrez gene record
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Pubmed identfication
Gene info
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Identity
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Gene
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Long gene nameRNA, U2 small nuclear 1
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Synonyms
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- U2 small nuclear ribonucleoprotein
- Small nuclear RNAs
Gene info
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Identity
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Gene
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Long gene namemajor histocompatibility complex, class I, U (pseudogene)
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Synonyms gene
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Synonyms gene name
- HLA-21 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2004-01-16
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Entrez gene record
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RefSeq identity
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Classification
- Histocompatibility complex
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameurotensin 2
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2000-03-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Receptor ligands
- Neuropeptides
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameRNA, U2 small nuclear 5, pseudogene
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Synonyms gene
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Synonyms gene name
- RNA, U2 small nuclear pseudogene 3
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-12-14
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Entrez gene record
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Pubmed identfication
Gene info
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Identity
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Gene
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Long gene namecentromere protein U
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Synonyms gene
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Synonyms gene name
- MLF1 interacting protein
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2003-07-17
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Constitutive centromere associated network
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameprotein tyrosine phosphatase receptor type O
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1995-02-22
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Entrez gene record
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Pubmed identfication
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Classification
- Protein tyrosine phosphatases receptor type
- Fibronectin type III domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemicroRNA 1289-2
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Synonyms gene
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Synonyms
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Locus
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Discovery year2008-11-03
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR1289 family