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Abbreviation
SW1116
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Leadtime
3-5 days
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Shipping condition
Dry ice
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Storage
Liquid nitrogen -196°C
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Age
73 years
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Morphology
Epithelial
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Growth_properties
Adherent
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Doubling_time
NA
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Background
CSAp negative(CSAp-).Colon antigen 3, negative.The cells are positive for keratin by immunoperoxidase staining.The line is positive for expression of c-myc, K-ras, H-ras, myb, sis and fos oncogenes.N-myc and N-ras oncogene expression were not detected.Tumor specific nuclear matrix proteins CC-4, CC-5 and CC-6 are expressed.
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Biosafety
1
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Medium
DMEM (PM150210)+10% FBS (164210-500)+1% P/S (PB180120)
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Subculturing
Cells must be subcultured at about 80% confuency , before they reach 90%. Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
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Ratio
1:2-1:4
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Renewal
Every 2 to 3 days
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Cryopreservation
Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
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Culture_conditions
Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
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Tumorigenic
Yes
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Effects
Yes, in nude mice. Tumors developed within 21 days at 100% frequency(5/5) in nude mice inoculated subcutaneously with 10^7 cells.
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Receptor_expression
NA
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Antigen_expression
Blood Type O; Rh＋
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Gene_expression
Carcinoembryonic Antigen(Cea) 2654Ng/10^6 Cells/10 Days; Keratin
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Applications
NA
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Duration
NA
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Description
For cells, cell lines and tissues in culture till half confluency.
