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Abbreviation
SV40 MES 13
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Leadtime
3-5 days
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Shipping condition
Dry ice
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Storage
Liquid nitrogen -196°C
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Age
7 to 10 weeks
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Morphology
Myofibroblast-like
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Growth_properties
Adherent
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Doubling_time
NA
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Background
The cells form colonies in soft agar, and are positive for SV40 large T antigen.The cells display prominent cytoskeletal staining for actin, and have abundant parallel fibrils in the cytoplasm. They are reported to contract in the presence of 1×10^-6 M angiotensin Ⅱ. The cells form colonies in soft agar, and are positive for SV40 large T antigen.
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Biosafety
2
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Medium
71.25% DMEM (PM150210)+23.75% F12 (PM150810)+5% FBS (164210-500)+1% P/S (PB180120)
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Subculturing
Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
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Ratio
1:2-1:4
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Renewal
Every 2 to 3 days
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Cryopreservation
Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
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Culture_conditions
Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
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Tumorigenic
NA
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Effects
Yes, forms colonies in soft agar.
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Receptor_expression
NA
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Antigen_expression
NA
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Gene_expression
NA
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Applications
NA
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Duration
NA
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Description
For cells, cell lines and tissues in culture till half confluency.
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Buiffer
MES buffers are Morpholine Ethane Sulphonic Acid or CAS 4432-31 buffers with compound 2-(N-morpholino)ethane sulfonic acid. Its chemical structure contains a morpholine ring. It has a molecular weight of 195.2 g/mol and the chemical formula is C6H13NO4S. Synonyms include 2-morpholinoethanesulfonic acid; 2-(4-morpholino)ethane sulfonic acid; 2-(N-morpholino)ethane sulfonic acid; 2-(4-morpholino)ethane sulfonic acid; MES; MES hydrate; and morpholine-4-ethanesulfonic acid hydrate. MOPS is a similar pH buffering compound which contains a propane sulfonic moiety instead of an ethane sulfonic one.
