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Abbreviation
Renca [RenCa]
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Leadtime
3-5 days
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Shipping condition
Dry ice
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Storage
Liquid nitrogen -196°C
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Age
6 weeks
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Morphology
Epithelial-like
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Growth_properties
Adherent
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Doubling_time
24h
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Background
The pattern of growth of this tumor accurately mimics that of human adult renal cell carcinoma, particularly with regard to spontaneous metastasis to lung and liver. The cells do not express transforming growth factor-beta type II receptor (TbetaR-II).
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Biosafety
1
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Medium
RPMI-1640 (PM150110)+0.1mM NEAA (PB180424)+1mM Sodium Pyruvate (PB180422)+2mM L-glutamine (PB180420)+10% FBS (164210-500)+1% P/S (PB180120)
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Subculturing
Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
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Ratio
1:2-1:4
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Renewal
Every 2 to 3 days
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Cryopreservation
Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
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Culture_conditions
Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃
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Tumorigenic
Yes
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Effects
NA
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Receptor_expression
NA
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Antigen_expression
NA
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Gene_expression
NA
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Applications
NA
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Duration
NA
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Description
For cells, cell lines and tissues in culture till half confluency.
