Recombinant Rat TrkA / NTRK1 Protein (His tag)
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Catalog number
PKSR030180-50µg
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Price
Please ask
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Size
50µg
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Synonym
NTRK1;Trk;Trka
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Activity
Measured by its ability to inhibit NGF-induced proliferation of TF‑1 human erythroleukemic cells.The ED50 for this effect is typically 0.2-0.8 µg/mL in the presence of 10 ng/mL of recombinant mouse NGF.
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Sequence
Met1-Pro418
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Fusion tag
C-His
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Accession
NA
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ExpressedHost
HEK293 Cells
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Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
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Purity
> 95 % as determined by SDS-PAGE
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Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
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Stability and Storage
Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Mol Mass
43.7 kDa
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AP Mol Mass
67-77 KDa
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Formulation
Lyophilized from sterile PBS, pH 7.4
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Background
TRKA is a member of the neurotrophic tyrosine kinase receptor (NTKR) family. It is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Isoform TrkA-III promotes angiogenesis and has oncogenic activity when overexpressed. Isoform TrkA-I is found in most non-neuronal tissues. Isoform TrkA-II is primarily expressed in neuronal cells. TrkA-III is specifically expressed by pluripotent neural stem and neural crest progenitors. The presence of NTRK1 leads to cell differentiation and may play a role in specifying sensory neuron subtypes. Mutations in TRKA gene have been associated with congenital insensitivity to pain, anhidrosis, self-mutilating behavior, mental retardation and cancer. It was originally identified as an oncogene as it is commonly mutated in cancers, particularly colon and thyroid carcinomas. TRKA is required for high-affinity binding to nerve growth factor (NGF), neurotrophin-3 and neurotrophin-4/5 but not brain-derived neurotrophic factor (BDNF). Known substrates for the Trk receptors are SHC1, PI 3-kinase, and PLC-gamma-1. NTRK1 has a crucial role in the development and function of the nociceptive reception system as well as establishment of thermal regulation via sweating. It also activates ERK1 by either SHC1- or PLC-gamma-1-dependent signaling pathway. Defects in NTRK1 are a cause of congenital insensitivity to pain with anhidrosis and thyroid papillary carcinoma.Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy
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Conjugation
histidine
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About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin name
Rattus norvegicus
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Source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
NTRK1
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Short name
Recombinant TrkA / NTRK1 Protein (His tag)
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
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Host
Rat
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Label
His
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Species
Rat, Rats
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Alternative name
Rec. Rat TrkA / neurotrophic tyrosine kinase, receptor, classification 1 Protein (histidine detection labelled)
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Alternative technique
rec
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Alternative to gene target
neurotrophic tyrosine kinase, receptor, type 1, MTC and p140-TrkA and TRK and Trk-A and TRK1 and TRKA, NTRK1 and IDBG-103632 and ENSG00000198400 and 4914, transferase activity, Cell surfaces, Ntrk1 and IDBG-158646 and ENSMUSG00000028072 and 18211, BT.22873 and IDBG-631386 and ENSBTAG00000003060 and 353111
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Gene info
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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