Recombinant Rat SerpinA1 Protein (His Tag) 50µg

• Catalog number
  PKSR030157-50µg
• Price
  Please ask
• Size
  50µg

• Synonym
  SerpinA1
• Activity
  NA
• Sequence
  Met1-Arg411
• Fusion tag
  C-His
• Accession
  NP_071964.2
• ExpressedHost
  HEK293 Cells
• Shipping
  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
• Purity
  > 95 % as determined by SDS-PAGE.
• Endotoxin
  < 1.0 EU per μg protein as determined by the LAL method.
• Stability and Storage
  Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Mol Mass
  45.2 kDa
• AP Mol Mass
  45.2 kDa
• Formulation
  Lyophilized from sterile PBS, pH 7.4
• Background
  SerpinA1, also known as Alpha-1 antitrypsin (AAT), is a prototype member of the Serpin superfamily of the serine protease inhibitors. This serine protease inhibitor blocks the protease, neutrophil elastase. Alpha-1 antitrypsin is mainly produced in the liver and acts as an antiprotease. Its principal function is to inactivate neutrophil elastase, preventing tissue damage. SerpinA1 (alpha1-antitrypsin), an acute phase protein and the classical neutrophil elastase inhibitor, is localized within lipid rafts in primary human monocytes in vitro. It association with monocytes is inhibited by cholesterol depleting/efflux-stimulating agents (nystatin, filipin, MbetaCD (methyl-beta-cyclodextrin) and oxidized low-density lipoprotein (oxLDL) and conversely, enhanced by free cholesterol. Furthermore, SerpinA1/monocyte association per se depletes lipid raft cholesterol as characterized by the activation of extracellular signal-regulated kinase 2, formation of cytosolic lipid droplets, and a complete inhibition of oxLDL uptake by monocytes. Previous population studies have suggested that heterozygote status for the AAT gene (SerpinA1) is a risk factor for chronic rhinosinusitis with nasal polyposis (CRSwNP). Alpha-1 antitrypsin deficiency is a recently identified genetic disease that occurs almost as frequently as cystic fibrosis. It is caused by various mutations in the SerpinA1 gene, and has numerous clinical implications. Alpha-1 antitrypsin deficiency is an inherited disease affecting the lung and liver. In the liver, alpha-1 antitrypsin deficiency may manifest as benign neonatal hepatitis syndrome; a small percentage of adults develop liver fibrosis, with progression to cirrhosis and hepatocellular carcinoma. Its most important physiologic functions are the protection of pulmonary tissue from aggressive proteolytic enzymes and regulation of pulmonary immune processes.
• Technical Data
• Conjugation
  histidine
• About
  Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
• Latin name
  Rattus norvegicus
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  SerpinA1   Protein   His   Tag   50µg  
• Short name
  Recombinant SerpinA1 Protein (His Tag) 50µg
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
• Host
  Rat
• Label
  His
• Species
  Rat, Rats
• Alternative name
  Rec. Rat serpin peptidase inhibitor, clade A (a-1 antiproteinase, antitrypsin), member 1 Protein (histidine detection labelled) 50µg
• Alternative technique
  rec
• Alternative to gene target
  serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1, A1A and A1AT and AAT and alpha1AT and PI and PI1 and PRO2275, SERPINA1 and IDBG-18174 and ENSG00000197249 and 5265, identical protein binding, Extracellular, Serpina1f and IDBG-167371 and ENSMUSG00000021081 and 68348, SERPINA1 and IDBG-634001 and ENSBTAG00000018843 and 280699

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

Similar products