Recombinant Rat CD111 / Nectin-1 / PVRL1 Protein (His tag)
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Catalog number
PKSR030290-50µg
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Price
Please ask
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Size
50µg
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Synonym
PVRL1
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Activity
NA
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Sequence
Met1-Ala354
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Fusion tag
C-His
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Accession
XP_236210.3
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ExpressedHost
HEK293 Cells
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Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
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Purity
> 97 % as determined by SDS-PAGE
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Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
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Stability and Storage
Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Mol Mass
37.6 kDa
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AP Mol Mass
55-60 KDa
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Formulation
Lyophilized from sterile PBS, pH 7.4
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Background
Poliovirus receptor-related 1 (herpesvirus entry mediator C; nectin-1; CD111), also known as PVRL1 is a cell adhesion molecule belonging to the immunoglobulin superfamily that can bind to virion glycoprotein D (gD) to mediate entry of herpes simplex viruses (HSV) and pseudorabies virus (PRV). CD111/Nectin-1/PVRL1 colocalizes with E-cadherin at adherens junctions in epithelial cells. The disruption of cell junctions can result in the redistribution of nectin-1. To determine whether disruption of junctions by calcium depletion influenced the susceptibility of epithelial cells to viral entry, Madin-Darby canine kidney cells expressing endogenous nectin-1 or transfected human nectin-1 were tested for the ability to bind soluble forms of viral gD and to be infected by HSV and PRV, before and after calcium depletion. It has been revealed that binding of HSV and PRV gD was localized to adherens junctions in cells maintained in normal medium but was distributed, along with nectin-1, over the entire cell surface after calcium depletion. Both the binding of gD and the fraction of cells that could be infected by HSV-1 and PRV were enhanced by calcium depletion. Taken together, CD111/Nectin-1/PVRL1 confined to adherens junctions in epithelial cells is not very accessible to virus, whereas dissociation of cell junctions releases nectin-1 to serve more efficiently as an entry recptor.
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Conjugation
histidine
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About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin name
Rattus norvegicus
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Source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
NECTIN1
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Short name
Recombinant CD111 / Nectin-1 / PVRL1 Protein (His tag)
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
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Host
Rat
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Label
His
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Species
Rat, Rats
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Alternative name
Rec. Rat CD111 / Nectin-1 / poliovirus receptor-related 1 (herpesvirus entry mediator C) Protein (histidine detection labelled)
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Alternative technique
rec
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Alternative to gene target
poliovirus receptor-related 1 (herpesvirus entry mediator C), CD111 and CLPED1 and ED4 and HIgR and HV1S and HVEC and nectin-1 and OFC7 and PRR and PRR1 and PVRR and PVRR1 and SK-12, PVRL1 and IDBG-74533 and ENSG00000110400 and 5818, cell adhesion molecule binding, Extracellular, Pvrl1 and IDBG-155297 and ENSMUSG00000032012 and 58235, PVRL1 and IDBG-631966 and ENSBTAG00000015939 and 533492
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Gene info
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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