Recombinant Mouse Programmed Cell Death 1 Ligand 1 Protein 50µg
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Catalog number
PKSM040919-50µg
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Price
Please ask
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Size
50µg
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Activity
Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse PD1-L1 at 1 μg/ml (100 μl/well) can bind mouse PD1 with a linear range of 6.25-400 ng/ml.
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Synonym
Programmed cell death 1 ligand 1Cd274; programmed cell death 1 ligand 1;PD-L1;PDCD1 ligand 1;programmed death ligand 1;B7 homolog 1;B7-H1;CD274
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Sequence
Met 1-Thr 238
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Fusion tag
C-6His
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Accession
NP_068693.1
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Expressed Host
Human Cells
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Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
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Purity
>95% as determined by reducing SDS-PAGE.
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Stability and Storage
Lyophilized protein should be stored at < -20℃, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7℃ for 2-7 days.Aliquots of reconstituted samples are stable at < -20℃ for 3 months
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Mol Mass
25.6 kDa
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AP Mol Mass
39-58 kDa
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Formulation
Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
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Background
Mouse Programmed cell death 1 ligand 1(Cd274,PD-L1), is a member of the growing B7 family of immune proteins.It involved in the costimulatory signal essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production.B7-H1 has been identified as one of two ligands for programmed death1 (PD1), a member of the CD28 family of immunoreceptors. B7-H1 is constitutively expressed in several organs such as heart, skeletal muscle B7-H1 expression is upregulated in a small fraction of activated T and B cells and a much larger fraction of activated monocytes. The costimulatory function of B7-H1 is critical for enhancing maturation and differentiation of T-cells in lymphoid organs.B7-H1 expression is also induced in dendritic cells and keratinocytes after IFN gamma stimulation. Interaction of B7-H1 with PD1 results in inhibition of TCR-mediated proliferation and cytokine production. The B7-H1:PD1 pathway is involved in the negative regulation of some immune responses and may play an important role in the regulation of peripheral tolerance.
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Description
For cells, cell lines and tissues in culture till half confluency. FAS ligand and other ligands are binding to the receptor for signaling pathways for example in apoptosis or JNK signaling. Receptor agonists are often tested for drug development.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Short name
Recombinant Mouse Programmed Death 1 Ligand 1 Protein 50µg
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Technique
Recombinant, Mouse, mouses, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Host
mouse
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Species
Mouse, Mouses
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Alternative name
Rec. Mouse Programmed cellular Death 1 Ligand 1 Protein 50µg
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Alternative technique
rec, murine
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Tissue
cell
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MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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