Recombinant Mouse ITK Kinase Protein (aa 351-619, His & GST tag)
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Catalog numberPKSM040718-20µg
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Price:
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Size20µg
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SynonymEmt;Tcsk;Tsk
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ActivityNA
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SequenceArg 351-Leu 619
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Fusion tagN-His & GST
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AccessionQ03526
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Expressed HostBaculovirus-Insect Cells
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ShippingIn general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
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Purity> 80 % as determined by SDS-PAGE
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Endotoxin< 1.0 EU per μg of the protein as determined by the LAL method
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Stability and StorageSamples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Mol Mass58.4 kDa
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AP Mol Mass58 kDa
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FormulationLyophilized from sterile 50mM Tris, 100mM NaCl, pH 7.5, 10% gly, 0.5mM GSH
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BackgroundIL-2-inducible T cell kinase is a member of the protein kinase superfamily, Tyr protein kinase family and TEC subfamily. It contains 1 Btk-type zinc finger, 1 PH domain, 1 protein kinase domain, 1 SH2 domain and 1 SH3 domain. As an intracellular kinase which expressed in T-cells, IL-2-inducible T cell kinase contains both SH2 and SH3 domains which are often found in intracellular kinases. It is hought to play a role in T-cell proliferation and differentiation. It regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. IL-2-inducible T cell kinase also plays an essential role in regulation of the adaptive immune response. efects in IL-2-inducible T cell kinase are the cause of lymphoproliferative syndrome EBV-associated autosomal type 1 (LPSA1). LPSA1 is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Inadequate immune response to EBV can have a fatal outcome. Clinical features include splenomegaly, lymphadenopathy, anemia, thrombocytopenia, pancytopenia, recurrent infections. There is an increased risk for lymphoma.
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Conjugationhistidine
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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SourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolMGST3, ITK, MIR619
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Short nameRecombinant Mouse ITK Kinase Protein (aa 351-619, His & GST tag)
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TechniqueRecombinant, Mouse, mouses, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
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Hostmouse
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LabelHis
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SpeciesMouse, Mouses
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Alternative nameRec. Mouse IL2-inducible T-cell kinase phosphorylation catalyst Protein (aa 351-619, histidine & GST detection labelled)
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Alternative techniquerec, murine
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Alternative to gene targetIL2-inducible T-cell kinase, EMT and LPFS1 and LYK and PSCTK2, ITK and IDBG-55348 and ENSG00000113263 and 3702, transferase activity, Cell surfaces, Itk and IDBG-165583 and ENSMUSG00000020395 and 16428, ITK and IDBG-646384 and ENSBTAG00000000306 and 522311
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Gene info
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Identity
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Gene
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Long gene namemicrosomal glutathione S-transferase 3
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1997-10-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Microsomal glutathione S-transferases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameIL2 inducible T cell kinase
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Synonyms gene name
- IL2-inducible T-cell kinase
- IL2 inducible T-cell kinase
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Synonyms
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GenBank acession
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Locus
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Discovery year1995-12-18
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Entrez gene record
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Pubmed identfication
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Classification
- Tec family tyrosine kinases
- SH2 domain containing
- Pleckstrin homology domain containing
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namemicroRNA 619
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Synonyms gene
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Synonyms
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Locus
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Discovery year2006-05-17
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNAs
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data