Recombinant Mouse EphB1 / EPHT2 Protein (His & GST tag)
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Catalog numberPKSM040302-20µg
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PricePlease ask
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Size20µg
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Synonym9330129L11;AW488255;C130099E04Rik;Cek6;Elk;Elkh;ENSMUSG00000074119;Hek6;Net
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ActivityThe specific activity was determined to be 234 nmol/min/mg using Poly(Glu,Tyr) 4:1 as substrate.
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SequenceMet 591-Ala 984
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Fusion tagN-His & GST
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AccessionQ8CBF3-1
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Expressed HostBaculovirus-Insect Cells
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ShippingKinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.Shipment made at ambient temperature may seriously affect the activity of the ordered products.
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Purity> 80 % as determined by SDS-PAGE
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Endotoxin< 1.0 EU per μg of the protein as determined by the LAL method
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Stability and StorageSamples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Mol Mass72.4 kDa
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AP Mol Mass60 kDa
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FormulationSupplied as sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% gly
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BackgroundEphrin type-B receptor 1, also known as EphB1, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. EphB2 receptor tyrosine kinase phosphorylates syndecan-2 and that this phosphorylation event is crucial for syndecan-2 clustering and spine formation. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. EphB receptor tyrosine kinases are enriched at synapses, suggesting that these receptors play a role in synapse formation or function. We find that EphrinB binding to EphB induces a direct interaction of EphB with NMDA-type glutamate receptors. This interaction occurs at the cell surface and is mediated by the extracellular regions of the two receptors, but does not require the kinase activity of EphB.
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Conjugationhistidine
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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SourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolEPHB1, MGST3
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Short nameRecombinant Mouse EphB1 / EPHT2 Protein (His & GST tag)
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TechniqueRecombinant, Mouse, mouses, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
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Hostmouse
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LabelHis
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SpeciesMouse, Mouses
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Alternative nameRec. Mouse EphB1 / EPHT2 Protein (histidine & GST detection labelled)
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Alternative techniquerec, murine
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Gene info
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Identity
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Gene
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Long gene nameEPH receptor B1
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Synonyms gene
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Synonyms gene name
- EphB1
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Synonyms
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GenBank acession
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Locus
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Discovery year1995-03-23
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Sterile alpha motif domain containing
- EPH receptors
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemicrosomal glutathione S-transferase 3
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1997-10-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Microsomal glutathione S-transferases
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data