Recombinant Mouse CD27 / TNFRSF7 Protein (His & Fc tag)

• Catalog number
  PKSM040860-50µg
• Price
  Please ask
• Size
  50µg

• Synonym
  S152;Tnfrsf7;Tp55
• Activity
  Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse CD27 at 2 μg/ml (100 μl/well) can bind biotinylated human CD70 with a linear range of 0.39-12.5 ng/ml.
• Sequence
  Met 1-Arg 182
• Fusion tag
  C-His & Fc
• Accession
  NP_001028298.1
• Expressed Host
  HEK293 Cells
• Shipping
  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
• Purity
  > 94 % as determined by SDS-PAGE
• Endotoxin
  < 1.0 EU per μg of the protein as determined by the LAL method
• Stability and Storage
  Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Mol Mass
  46.2 kDa
• AP Mol Mass
  60-65 kDa
• Formulation
  Lyophilized from sterile PBS, pH 7.4
• Background
  CD27, also known as TNFRSF7, is a member of the TNF-receptor superfamily limited to cells of the lymphoid lineage, and exists as both a dimeric glycoprotein on the cell surface and as a soluble protein in serum. As a type I transmembrane glycoprotein of about 55 kDa existing as disulfide-linked homodimer, CD27 has been shown to play roles in lymphoid proliferation, differentiation, and apoptosis.It has important role in generation of T cell immunity, and is an apparently robust marker for normal memory B cells. It is a T and B cell co-stimulatory molecule, the activity of CD27 is governed by its TNF-like ligand CD70 on lymphocytes and dendritic cells. The CD27-CD70 interaction is required for Th1 generation responses to differentiation signals and long-term maintenance of T cell immunity, and meanwhile, plays a key role in regulating B-cell differentiation, activation and immunoglobulin synthesis.Immune CheckpointImmune Checkpoint Detection: Antibodies   Immune Checkpoint Detection: ELISA Antibodies   Immune Checkpoint Detection: IHC Antibodies   Immune Checkpoint Detection: ICC Antibodies   Immune Checkpoint Detection: FCM AntibodiesImmune Checkpoint ProteinsImmune Checkpoint Targets   Co-stimulatory Immune Checkpoint Targets Immunotherapy   Cancer Immunotherapy   Targeted Therapy
• Technical data
• Conjugation
  histidine
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  CD27   TNFRSF7   Protein   His   tag  
• Gene symbol
  CD27, CD27-AS1
• Short name
  Recombinant Mouse CD27 / TNFRSF7 Protein (His & Fc tag)
• Technique
  Recombinant, Mouse, FC, mouses, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
• Host
  mouse
• Label
  His
• Species
  Mouse, Mouses
• Alternative name
  Rec. Mouse CD27 molecule / TNFRSF7 Protein (histidine & fragment c detection labelled)
• Alternative technique
  rec, murine
• Alternative to gene target
  CD27 molecule, S152 and S152. LPFS2 and T14 and TNFRSF7 and Tp55, CD27 and IDBG-14014 and ENSG00000139193 and 939, cysteine-type endopeptidase inhibitor activity involved in apoptotic process, Extracellular, Cd27 and IDBG-189750 and ENSMUSG00000030336 and 21940, CD27 and IDBG-640494 and ENSBTAG00000014725 and 512514

Gene info

• Identity
  HGNC:11922
• Gene
  CD27
• Long gene name
  CD27 molecule
• Synonyms gene
  • TNFRSF7
• Synonyms gene name
  • tumor necrosis factor receptor superfamily, member 7
• Synonyms
  • S152
  • Tp55
• GenBank acession
  • M63928
• Locus
  12p13.31
• Discovery year
  1992-06-10
• Entrez gene record
  939
• Pubmed identfication
  • 2442250
  • 8530100
• Classification
  • Tumor necrosis factor receptor superfamily
  • CD molecules
• VEGA ID
  OTTHUMG00000168316
• Locus Specific Databases
  • LRG_357

Gene info

• Identity
  HGNC:43896
• Gene
  CD27-AS1
• Long gene name
  CD27 antisense RNA 1
• Synonyms gene name
  • CD27 antisense RNA 1 (non-protein coding)
• GenBank acession
  • AK056500
• Locus
  12p13.31
• Discovery year
  2012-04-27
• Entrez gene record
  678655
• RefSeq identity
  • NR_015382
• Classification
  • Antisense RNAs
• VEGA ID
  OTTHUMG00000168315

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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