Recombinant Mouse BLMH / Bleomycin Hydrolase Protein (His tag)

• Catalog number
  PKSM040554-10µg
• Price
  Please ask
• Size
  10µg

• Synonym
  AI035728;Bh;Bmh
• Activity
  Measured by its ability to hydrolyze Met-AMC. The specific activity is >500 pmoles/min/μg.
• Sequence
  Asn 2-Glu 455
• Fusion tag
  N-His
• Accession
  NP_848760.1
• Expressed Host
  E.coli
• Shipping
  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
• Purity
  > 95 % as determined by SDS-PAGE
• Endotoxin
  Please contact us for more information.
• Stability and Storage
  Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Mol Mass
  53.3 kDa
• AP Mol Mass
  47 kDa
• Formulation
  Lyophilized from sterile 50mM Tris, 0.15M NaCl, 10% glycerol, pH 8.0
• Background
  The papain superfamily member bleomycin hydrolase (BLMH) is a cytoplasmic cysteine peptidase that is highly conserved through evolution. The only known activity of the enzyme is metabolic inactivation of the glycopeptide bleomycin (BLM), an essential component of combination chemotherapy regimens for cancer. The papain superfamily member bleomycin hydrolase (BLMH) is a neutral cysteine protease with structural similarity to a 20S proteasome. Bleomycin (BLM), a clinically used glycopeptide anticancer agent. BLMH is an essential protectant against BLM-induced death and has an important role in neonatal survival and in maintaining epidermal integrity. Sequencing revealed several putative sites phosphorylated by different types of protein kinases, but no signal sequence, transmembrane domain, N-linked glycosylation site or DNA-binding motif.
• Technical data
• Conjugation
  histidine
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  BLMH   Bleomycin   Hydrolase   Protein   His   tag  
• Gene symbol
  BLMH
• Short name
  Recombinant Mouse BLMH / Bleomycin Hydrolase Protein (His tag)
• Technique
  Recombinant, Mouse, mouses, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
• Host
  mouse
• Label
  His
• Species
  Mouse, Mouses
• Alternative name
  Rec. Mouse BLMH / Bleomycin Hydrolase Protein (histidine detection labelled)
• Alternative technique
  rec, murine

Gene info

• Identity
  HGNC:1059
• Gene
  BLMH
• Long gene name
  bleomycin hydrolase
• Synonyms
  • BH
• GenBank acession
  • X92106
• Locus
  17q11.2
• Discovery year
  1998-03-11
• Entrez gene record
  642
• Pubmed identfication
  • 9407121
  • 9331073
• RefSeq identity
  • NM_000386
• Classification
  • Peptidase family C1
• VEGA ID
  OTTHUMG00000179756

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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