Recombinant Human Ubiquitin Carboxyl-Terminal Hydrolase 14/USP14 Protein(N-6His)
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Catalog number
PKSH033174-10µg
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Price
Please ask
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Size
10µg
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Synonym
Ubiquitin Carboxyl-Terminal Hydrolase 14; Deubiquitinating Enzyme 14; Ubiquitin Thioesterase 14; Ubiquitin-Specific-Processing Protease 14; USP14; TGT
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Activity
NA
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Sequence
Asp91-Gln494
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Fusion tag
N-6His
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Accession
P54578
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Expressed Host
E.coli
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Shipping
The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.
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Purity
＞95% as determined by reducing SDS-PAGE.
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Endotoxin
＜1.0 EU per µg as determined by LAL test.
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Stability and Storage
Store at < -20℃, stable for 6 monthshs after receipt.Please minimize freeze-thaw cycles
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Mol Mass
48.5 kDa
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AP Mol Mass
52 kDa
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Formulation
Supplied as a 0.2 μm filtered solution of 20mM TrisHCl, 100mM NaCl, 20% Glycerol, pH 8.0.
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Background
Ubiquitin Carboxyl-Terminal Hydrolase 14 (USP14) belongs to the ubiquitin-specific processing (USP) family which is a deubiquitinating enzyme (DUB) with His and Cys domains. USP14 located in the cytoplasm is a proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. USP14 acts also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. In addition, USP14 is indispensable for synaptic development and function at neuromuscular junctions, required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
USP14
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Short name
Recombinant Ubiquitin Carboxyl-Terminal Hydrolase 14/USP14 Protein(N-6His)
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Human, Humans
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Alternative name
Rec. H. sapiens Ubiquitin Carboxyl-Terminal Hydrolase 14/ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase) Protein(N-6His)
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Alternative technique
rec
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Alternative to gene target
ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase), TGT, USP14 and IDBG-259 and ENSG00000101557 and 9097, proteasome binding, Cell surfaces, Usp14 and IDBG-128322 and ENSMUSG00000047879 and 59025, BT.20599 and IDBG-644085 and ENSBTAG00000019214 and 505106
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Gene info
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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