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Synonym
CD71;p90;T9;TFR;TFR1;TR;TRFR
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Activity
Measured by its binding ability in a functional ELISA. Immobilized human CD71 at 10 μg/ml (100 μl/well) can bind human Transferrin. The EC50 of human Transferrin is 5.6 ng/mL.
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Sequence
Cys 89-Phe 760
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Fusion tag
N-His
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Accession
NP_003225.2
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Expressed Host
HEK293 Cells
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Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
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Purity
>85 % as determined by SDS-PAGE
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Endotoxin
<1.0 EU per µg as determined by the LAL method
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Stability and Storage
Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Mol Mass
77.4 kDa
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AP Mol Mass
77.4 kDa
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Formulation
Lyophilized from sterile PBS, pH 7.4
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Background
Transferrin receptor protein 1, also known as transferrin receptor, Trfr, p90, CD71 and TFRC, is a single-pass type II membrane protein which belongs to the peptidase M28 family and M28B subfamily. TFRC / CD71 is a membrane-bound protein expressed in larger amounts in proliferating. The specific expression of TFRC can represent a diagnostic tool or a therapeutic target in solid tumours expressing this antigen. Transferrin receptor is necessary for development of erythrocytes and the nervous system. TFRC / CD71 is regulated by cellular iron levels through binding of the iron regulatory proteins, IRP1 and IRP2, to iron-responsive elements in the 3'-UTR. Up-regulated upon mitogenic stimulation. TFRC / CD71 represents a marker of malignant transformation in the pancreas that could be applied as potential diagnostic and therapeutic target.
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Conjugation
histidine
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Description
The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
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Source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
TFRC
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Short name
Recombinant Transferrin Receptor / TFRC / CD71 Protein (His tag)
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
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Label
His
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Species
Human, Humans
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Alternative name
Rec. H. sapiens Transferrin Receptor / transferrin receptor (p90, CD71) / CD71 Protein (histidine detection labelled)
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Alternative technique
rec
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Alternative to gene target
transferrin receptor (p90, CD71), CD71 and p90 and T9 and TFR and TFR1 and TR and TRFR, TFRC and IDBG-71016 and ENSG00000072274 and 102724492,7037, poly(A) RNA binding, Extracellular, Tfrc and IDBG-154429 and ENSMUSG00000022797 and 22042, TFRC and IDBG-633841 and ENSBTAG00000032719 and 100852305,504698
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