Recombinant Human TFPI Protein (His tag)

• Catalog number
  PKSH031525-10µg
• Price
  Please ask
• Size
  10µg

• Synonym
  EPI;LACI;TFI;TFPI1
• Activity
  Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Anaspec, Catalog#27114). The IC50 value is < 0.4 nM, as measured in 100μL reaction mixture containing 1.25 ng trypsin (Sigma, Catalog#T4799), 10 μM substrate, 50 mM Tris, 10 mM CaCl2, 0.15 M NaCl, 0.05% Brij-35, pH 7.5.
• Sequence
  Met 1-Lys 282
• Fusion tag
  C-His
• Accession
  NP_006278.1
• Expressed Host
  HEK293 Cells
• Shipping
  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
• Purity
  ＞85 % as determined by SDS-PAGE
• Endotoxin
  ＜1.0 EU per µg as determined by the LAL method
• Stability and Storage
  Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Mol Mass
  30.6 kDa
• AP Mol Mass
  42-45 kDa
• Formulation
  Lyophilized from sterile PBS, pH 7.4
• Background
  Tissue factor pathway inhibitor (TFPI) is the natural inhibitor of TF coagulant and signaling activities. It is a Kunitz-type serine proteinase inhibitor that down-regulates tissue factor-initiated blood coagulation. With its Kunitz domains, TFPI exhibits significant homology with human inter-alpha-trypson inhibitor and bovin basic pancreatic trypsin inhibitor. TFPI is the natural inhibitor of TF coagulant and signaling activities. The importance of TFPI in the regulation of blood coagulation is emphasized by how its activity is modulated in human disease. In a factor (F) Xa-dependent feedback system, TFPI binds directly and inhibits the TF-FVII/FVIIa complex. Normally, TFPI exists in plasma both as a full-length molecule and as variably carboxy-terminal truncated forms. TFPI also circulates in complex with plasma lipoproteins. The levels and the dual inhibitor effect of TFPI on FXa and TF-FVII/FVIIa complex offers insight into the mechanisms of various pathological conditions triggered by TF. TFPI may play an important role in modulating TF-induced thrombogenesis and it may also provide a unique therapeutic approach for prophylaxis and/or treatment of various diseases. In addition, Studies have shown that TFPI exhibits antiangiogenic and antimetastatic effects in vitro and in vivo. In animal models of experimental metastasis, both circulating and tumor cell-associated TFPI are shown to significantly reduce tumor cell-induced coagulation activation and lung metastasis.
• Technical data
• Conjugation
  histidine
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  TFPI   Protein   His   tag  
• Gene symbol
  TFPI2, TFPI
• Short name
  Recombinant TFPI Protein (His tag)
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
• Label
  His
• Species
  Human, Humans
• Alternative name
  Rec. H. sapiens TFPI Protein (histidine detection labelled)
• Alternative technique
  rec

Gene info

• Identity
  HGNC:11761
• Gene
  TFPI2
• Long gene name
  tissue factor pathway inhibitor 2
• Synonyms
  • PP5
  • TFPI-2
  • REF1
• GenBank acession
  • L27624
• Locus
  7q21.3
• Discovery year
  1999-07-14
• Entrez gene record
  7980
• Pubmed identfication
  • 7896752
  • 8945635
• RefSeq identity
  • NM_006528
• VEGA ID
  OTTHUMG00000022963
• Locus Specific Databases
  • LRG_647

Gene info

• Identity
  HGNC:11760
• Gene
  TFPI
• Long gene name
  tissue factor pathway inhibitor
• Synonyms gene
  • LACI
• Synonyms gene name
  • tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor)
• Synonyms
  • EPI
  • TFI
  • TFPI1
• Synonyms name
  • extrinsic pathway inhibitor
• Locus
  2q32.1
• Discovery year
  1991-06-03
• Entrez gene record
  7035
• Pubmed identfication
  • 1993173
• RefSeq identity
  • NM_006287
• VEGA ID
  OTTHUMG00000132634
• Locus Specific Databases
  • LRG_604

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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