Recombinant Human Semaphorin 4A / SEMA4A / Semaphorin B Protein (His tag) 100µg

• Catalog number
  PKSH031007-100µg
• Price
  Please ask
• Size
  100µg

• Synonym
  CORD10;RP35;SEMAB;SEMB
• Activity
  Measured by its ability to bind biotinylated mouse SEMA4D in a functional ELISA.
• Sequence
  Met 1-His 683
• Fusion tag
  C-His
• Accession
  NP_071762.2
• Expressed Host
  HEK293 Cells
• Shipping
  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
• Purity
  ＞96 % as determined by SDS-PAGE
• Endotoxin
  ＜1.0 EU per µg as determined by the LAL method
• Stability and Storage
  Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Mol Mass
  73.4 kDa
• AP Mol Mass
  75-80 kDa
• Formulation
  Lyophilized from sterile PBS, pH 7.4
• Reconstitution
  Please refer to it for detailed information.
• Background
  Semaphorin-4A, also known as Semaphorin-B, SEMA4A, Sema B and SEMAB, is a single-pass type I  membrane protein which belongs to the semaphorin family. It inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons. Semaphorin-4A / SEMA4A contains one Ig-like C2-type (immunoglobulin-like) domain, one PSI domain and one Sema domain. Defects in SEMA4A are the cause of retinitis pigmentosa type 35 (RP35) which leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. Defects in SEMA4A are also the cause of cone-rod dystrophy type 10 (CORD10) which are inherited retinal dystrophies belonging to the group of pigmentary retinopathies. CORDs are characterized by retinal pigment deposits visible on fundus examination, predominantly in the macular region, and initial loss of cone photoreceptors followed by rod degeneration. Semaphorins are secreted, transmembrane, and GPI-linked proteins, defined by cysteine-rich semaphorin protein domains, that have important roles in a variety of tissues. Humans have 20 semaphorins, Drosophila has five, and two are known from DNA viruses. Semaphorins are found in nematodes and crustaceans but not in non-animals. They are grouped into eight classes on the basis of phylogenetic tree analyses and the presence of additional protein motifs. Semaphorins have been implicated in diverse developmental processes such as axon guidance during nervous system development and regulation of cell migration.
• Product URL
• PDF URL
• Conjugation
  histidine
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Semaphorin   SEMA4A   Semaphorin   Protein   His   tag   100µg  
• Gene symbol
  SEMA4A
• Short name
  Recombinant Semaphorin 4A / SEMA4A / Semaphorin B Protein (His tag) 100µg
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
• Label
  His
• Species
  Human, Humans
• Alternative name
  Rec. H. sapiens Semaphorin 4A / sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A / Semaphorin B Protein (histidine detection labelled) 100µg
• Alternative technique
  rec
• Alternative to gene target
  sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A, SEMA4A and IDBG-103399 and ENSG00000196189 and 64218, protein binding, Plasma membranes, Sema4a and IDBG-160873 and ENSMUSG00000028064 and 20351, SEMA4A and IDBG-631665 and ENSBTAG00000012228 and 510239

Gene info

• Identity
  HGNC:10729
• Gene
  SEMA4A
• Long gene name
  semaphorin 4A
• Synonyms gene
  • SEMAB
• Synonyms gene name
  • sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A
• Synonyms
  • SemB
  • FLJ12287
  • CORD10
• GenBank acession
  • AK022416
• Locus
  1q22
• Discovery year
  2004-04-22
• Entrez gene record
  64218
• Pubmed identfication
  • 7748561
  • 12374982
• RefSeq identity
  • NM_022367
• Classification
  • Semaphorins
• VEGA ID
  OTTHUMG00000014042

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

Similar products