Recombinant Human PTP1B / PTPN1 Protein (His Tag)

• Catalog number
  PKSH030416-50µg
• Price
  Please ask
• Size
  50µg

• Synonym
  PTP1B;PTPN1
• Activity
  Measured by its ability to dephosphorylate a phosphotyrosine residue in an EGF receptor (aa988-998) phosphopeptide substrate, R&D Systems, Catalog # ES006.The specific activity is > 15 nmoles/min/μg.
• Sequence
  Glu 2-Asn 321
• Fusion tag
  N-His
• Accession
  NP_002818.1
• Expressed Host
  E. coli
• Shipping
  Liquid. It is shipped out with blue ice.
• Purity
  ＞95 % as determined by SDS-PAGE
• Endotoxin
  Please contact us for more information.
• Stability and Storage
  Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Mol Mass
  38 kDa
• AP Mol Mass
  38 kDa
• Formulation
  Supplied as sterile 10 mM Hepes, 150 mM NaCl, 1 mM DTT, pH 7.5
• Background
  PTP1B, also known as PTPN1, belongs to the protein-tyrosine phosphatase (PTP) family. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. PTP1B contains 1 tyrosine-protein phosphatase domain and is expressed in many tissues. PTP1B is localized to the cytoplasmic face of the endoplasmic reticulum. PTP1B was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of PTP1B in cell growth control, and cell response to IFN stimulation.
• Technical data
• Conjugation
  histidine
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  PTP1B   PTPN1   Protein   His   Tag  
• Gene symbol
  PTPN1
• Short name
  Recombinant PTP1B / PTPN1 Protein (His Tag)
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
• Label
  His
• Species
  Human, Humans
• Alternative name
  Rec. H. sapiens PTP1B / PTPN1 Protein (histidine detection labelled)
• Alternative technique
  rec

Gene info

• Identity
  HGNC:9642
• Gene
  PTPN1
• Long gene name
  protein tyrosine phosphatase non-receptor type 1
• Synonyms gene
  • PTP1B
• Locus
  20q13.13
• Discovery year
  1991-09-13
• Entrez gene record
  5770
• Pubmed identfication
  • 2164224
• Classification
  • Protein tyrosine phosphatases non-receptor type
• VEGA ID
  OTTHUMG00000032729

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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