Recombinant Human Pleiotrophin / PTN / HB-GAM Protein 20µg
-
Catalog number
PKSH031708-20µg
-
Price
Please ask
-
Size
20µg
-
-
Synonym
HARP;HBGF8;HBNF;NEGF1
-
Activity
Measured by its binding ability in a functional ELISA. Immobilized human PTN at 10 μg/ml (100 μl/well) can bind rat SDC1-Fc, The EC50 of rat SDC1-Fc is 0.35-0.81 μg/ml.
-
Sequence
Met1-Asp168
-
Fusion tag
NA
-
Accession
NP_002816.1
-
Expressed Host
Baculovirus-Insect Cells
-
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
-
Purity
＞90 % as determined by SDS-PAGE
-
Endotoxin
＜1.0 EU per µg as determined by the LAL method
-
Stability and Storage
Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Mol Mass
15.3 kDa
-
AP Mol Mass
19 kDa
-
Formulation
Lyophilized from sterile 20mM Tris, 1M NaCl, pH 8.0
-
Reconstitution
Please refer to it for detailed information.
-
Background
HB-GAM belongs to the pleiotrophin family. During embryonic and early postnatal development, HB-GAM is expressed in the central and peripheral nervous system and also in several non-neural tissues, notably lung, kidney, gut and bone. While in the adult central nervous system, it is expressed in an activity-dependent manner in the hippocampus where it can suppress long term potentiation induction. HB-GAM has a low expression in other areas of the adult brain, but it can be induced by ischemic insults, or targeted neuronal damaged in the entorhinal cortex or in the substantia nigra pars compacta. It is structurally related to midkine and retinoic acid induced heparin-binding protein and has a high affinity for heparin. HB-GAM binds anaplastic lymphoma kinase (ALK) which induces MAPK pathway activation, an important step in the anti-apoptotic signaling of PTN and regulation of cell proliferation. It also functions as a secreted growth factor and induces neurite outgrowth and which is mitogenic for fibroblasts, epithelial, and endothelial cells.
-
-
-
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
Source
Recombinants or rec. proteins
-
Group
recombinants
-
Gene target
-
Gene symbol
HMHB1, KRT84, PTN, KRT85, KRT81, KRT82, KRT83, PEG10
-
Short name
Recombinant Pleiotrophin / PTN / HB-GAM Protein 20µg
-
Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
-
Species
Human, Humans
-
Alternative name
Rec. H. sapiens Pleiotrophin / PTN / HB-GAM Protein 20µg
-
Alternative technique
rec
-
Gene info
Gene info
-
Identity
-
Gene
-
Long gene name
keratin 84
-
Synonyms gene
-
Synonyms gene name
- keratin, hair, basic, 4
- keratin 84, type II
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year
1998-04-21
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene name
pleiotrophin
-
Synonyms gene
-
Synonyms gene name
- neurite growth-promoting factor 1
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year
1993-06-22
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene name
keratin 85
-
Synonyms gene
-
Synonyms gene name
- keratin, hair, basic, 5
- keratin 85, type II
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year
1998-04-21
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene name
keratin 81
-
Synonyms gene
-
Synonyms gene name
- keratin, hair, basic, 1
- keratin 81, type II
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year
1998-04-21
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene name
keratin 82
-
Synonyms gene
-
Synonyms gene name
- keratin, hair, basic, 2
- keratin 82, type II
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year
1998-04-21
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene name
keratin 83
-
Synonyms gene
-
Synonyms gene name
- keratin, hair, basic, 3
- keratin 83, type II
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year
1998-04-21
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
-
VEGA ID
-
Locus Specific Databases
Gene info
-
Identity
-
Gene
-
Long gene name
paternally expressed 10
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year
2000-11-28
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
-
VEGA ID
-
Locus Specific Databases
MeSH Data
-
Name
-
Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
-
Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
Similar products
