Recombinant Human PDK / PDZ binding kinase / TOPK Protein (His tag)
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Catalog numberPKSH031457-20µg
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PricePlease ask
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Size20µg
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SynonymCT84;HEL164;Nori-3;SPK;TOPK
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ActivityNA
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SequenceMet 1-Val 322
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Fusion tagC-His
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AccessionNP_060962.2
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Expressed HostBaculovirus-Insect Cells
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ShippingIn general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
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Purity＞80 % as determined by SDS-PAGE
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Endotoxin＜1.0 EU per µg as determined by the LAL method
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Stability and StorageSamples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Mol Mass37 kDa
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AP Mol Mass37 kDa
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FormulationLyophilized from sterile PBS, pH 7.4
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BackgroundPDZ binding kinase (PBK), also known as TOPK (T-LAK cell-originated protein kinase), is a serine/threonine kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family, and has all the characteristic protein kinase subdomains and a C-terminal PDZ-binding T/SXV motif. PBK is expressed in the testis restrictedly expressed in outer cell layer of seminiferous tubules, as well as placenta. PBK may be enrolled in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis.This mitotic kinase phosphorylates MAP kinase p38 and seems to be active in mitosis. When phosphorylated, PBK forms a protein-protein interaction with tumor suppressor p53 (TP53), leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage. The expression level of PBK is thus upregulated in a variety of neoplasms including hematological malignancies.
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Conjugationhistidine
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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SourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolARHGEF11, RGS3, PBK, RAPGEF6, RAPGEF2, USH1C
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Short nameRecombinant PDK / PDZ binding kinase / TOPK Protein (His tag)
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
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LabelHis
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SpeciesHuman, Humans
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Alternative nameRec. H. sapiens PDK / PDZ binding phosphorylation catalyst / TOPK Protein (histidine detection labelled)
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Alternative techniquerec
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Gene info
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Identity
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Gene
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Long gene nameRho guanine nucleotide exchange factor 11
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Synonyms gene name
- Rho guanine nucleotide exchange factor (GEF) 11
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Synonyms
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GenBank acession
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Locus
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Discovery year2001-02-08
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- PDZ domain containing
- Dbl family Rho GEFs
- MicroRNA protein coding host genes
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameregulator of G protein signaling 3
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Synonyms gene name
- regulator of G-protein signalling 3
- regulator of G-protein signaling 3
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Synonyms
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GenBank acession
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Locus
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Discovery year1997-06-09
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Regulators of G-protein signaling
- C2 domain containing
- PDZ domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namePDZ binding kinase
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2005-01-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameRap guanine nucleotide exchange factor 6
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Synonyms gene
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Synonyms gene name
- PDZ domain containing guanine nucleotide exchange factor (GEF) 2
- Rap guanine nucleotide exchange factor (GEF) 6
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Synonyms
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GenBank acession
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Locus
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Discovery year2003-09-03
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- PDZ domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameRap guanine nucleotide exchange factor 2
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Synonyms gene
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Synonyms gene name
- PDZ domain containing guanine nucleotide exchange factor (GEF) 1
- Rap guanine nucleotide exchange factor (GEF) 2
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2003-05-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- MicroRNA protein coding host genes
- PDZ domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameUSH1 protein network component harmonin
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Synonyms gene
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Synonyms gene name
- deafness, autosomal recessive 18
- Usher syndrome 1C (autosomal recessive, severe)
- Usher syndrome 1C
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1992-06-08
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- PDZ domain containing
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
