Recombinant Human NME1 / NDKA Protein (His tag)

• Catalog number
  PKSH030357-50µg
• Price
  Please ask
• Size
  50µg

• Synonym
  AWD;GAAD;NB;NBS;NDKA;NDPK-A;NDPKA;NM23;NM23-H1
• Activity
  NA
• Sequence
  Ala 2-Glu 152
• Fusion tag
  N-His
• Accession
  NP_000260.1
• Expressed Host
  E. coli
• Shipping
  Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.Shipment made at ambient temperature may seriously affect the activity of the ordered products.
• Purity
  ＞98 % as determined by SDS-PAGE
• Endotoxin
  Please contact us for more information.
• Stability and Storage
  Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Mol Mass
  18 kDa
• AP Mol Mass
  21 kDa
• Formulation
  Supplied as sterile PBS, pH 7.4
• Background
  NME1, also known as Nucleoside Diphosphate Kinase A (NDK-A), or NM23-H1, belongs to the NDK family. NM23-H1 is known to have a metastasis suppressive activity in many tumor cells. Recent studies have shown that the interacting proteins with NM23-H1 which mediate the cell proliferation, may act as modulators of the metastasis suppressor activity. The interacting proteins with NM23-H1 can be classified into 3 groups. The first group of proteins can be classified as upstream kinases of NM23-H1 such as CKI and Aurora-A/STK15. The second group of proteins acts as downstream effectors for the regulation of specific gene transcriptions, GTP-binding protein functions, and signal transduction in Erk signal cascade. The third group of proteins can be classified as bi-directionally influencing binding partners of NM23-H1. As a result, the interactions with NM23-H1 and binding partners have implications in the biochemical characterization involved in metastasis and tumorigenesis. NDKA is increased in human postmortem cerebrospinal fluid (CSF), a model of global brain insult, suggesting that measurement in CSF and, more importantly, in plasma may be useful as a biomarker of stroke. Additionally, NM23-H1 significantly reduces metastasis without effects on primary tumor size and was the first discovered metastasis suppressor gene.
• Technical data
• Conjugation
  histidine
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  NME1   NDKA   Protein   His   tag  
• Gene symbol
  NME1-NME2, NME1, RMRP
• Short name
  Recombinant NME1 / NDKA Protein (His tag)
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
• Label
  His
• Species
  Human, Humans
• Alternative name
  Rec. H. sapiens NME/NM23 nucleoside diphosphate kinase 1 / NDKA Protein (histidine detection labelled)
• Alternative technique
  rec
• Alternative to gene target
  NME/NM23 nucleoside diphosphate kinase 1, AWD and GAAD and NB and NBS and NDKA and NDPK-A and NDPKA and NM23 and NM23-H1, NME1 and IDBG-409263 and ENSG00000239672 and 4830, poly(A) RNA binding, nuclei, Nme1 and IDBG-208565 and ENSMUSG00000037601 and 18102, NME1 and IDBG-638442 and ENSBTAG00000004651 and 404189

Gene info

• Identity
  HGNC:33531
• Gene
  NME1-NME2
• Long gene name
  NME1-NME2 readthrough
• Synonyms
  • NM23-LV
  • NMELV
• GenBank acession
  • DQ109675
• Locus
  17q21.33
• Discovery year
  2007-07-31
• Entrez gene record
  654364
• Pubmed identfication
  • 16442775
• VEGA ID
  OTTHUMG00000137475

Gene info

• Identity
  HGNC:7849
• Gene
  NME1
• Long gene name
  NME/NM23 nucleoside diphosphate kinase 1
• Synonyms gene name
  • non-metastatic cells 1, protein (NM23A) expressed in
• Synonyms
  • NM23
  • NM23-H1
  • NDPKA
• GenBank acession
  • AL360191
  • X17620
• Locus
  17q21.33
• Discovery year
  1991-07-26
• Entrez gene record
  4830
• Pubmed identfication
  • 8270257
  • 19852809
• RefSeq identity
  • NM_000269
• Classification
  • NME/NM23 family
  • SET complex
• VEGA ID
  OTTHUMG00000137474

Gene info

• Identity
  HGNC:10031
• Gene
  RMRP
• Long gene name
  RNA component of mitochondrial RNA processing endoribonuclease
• Synonyms gene
  • CHH
• Synonyms gene name
  • cartilage-hair hypoplasia
• Synonyms
  • RMRPR
  • RRP2
  • NME1
• GenBank acession
  • M29916
• Locus
  9p13.3
• Discovery year
  1989-06-30
• Entrez gene record
  6023
• Pubmed identfication
  • 11207361
  • 1623519
• RefSeq identity
  • NR_003051
• Locus Specific Databases
  • LRG_163

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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