Recombinant Human Myeloperoxidase/MPO Protein(C-10His)
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Catalog number
PKSH032773-10µg
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Price
Please ask
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Size
10µg
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Synonym
Myeloperoxidase; MPO
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Activity
NA
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Sequence
Ala49-Ser745
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Fusion tag
C-10His
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Accession
P05164
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Expressed Host
Human Cells
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Shipping
The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.
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Purity
＞90% as determined by reducing SDS-PAGE.
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Endotoxin
＜1.0 EU per µg as determined by LAL test.
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Stability and Storage
Lyophilized protein should be stored at < -20℃, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7℃ for 2-7 days.Aliquots of reconstituted samples are stable at < -20℃ for 3 months
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Mol Mass
80.3 kDa
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AP Mol Mass
85-95 kDa
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Formulation
Lyophilized from a 0.2 μm filtered solution of 20mM Tris, 150mM NaCl, pH8.0 .
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Background
Myeloperoxidase (MPO) is a heme-containing enzyme belonging to the XPO subfamily of peroxidases. It is an abundant neutrophil and monocyte glycoprotein that catalyzes the hydrogen peroxide-dependent conversion of chloride, bromide, and iodide to multiple reactive species. Post-translational processing of MPO involves the insertion of a heme moiety and the proteolytic removal of both a propeptide and a 6 aa internal peptide. This results in a disulfide-linked dimer composed of a 60 kDa heavy and 12 kDa light chain that associate into a 150 kDa enzymatically active tetramer. The tetramer contains two heme groups and one disulfide bond between the heavy chains. Alternate splicing generates two additional isoforms of MPO, one with a 32 aa insertion in the light chain, and another with a deletion of the signal sequence and part of the propeptide. Human and mouse MPO share 87% aa sequence identity. MPO activity results in protein nitrosylation and the formation of 3-chlorotyrosine and dityrosine crosslinks. MPO is also associated with a variety of other diseases, and inhibits vasodilation in inflammation by depleting the levels of NO. Serum albumin functions as a carrier protein during MPO movement to the basolateral side of epithelial cells. MPO is stored in neutrophil azurophilic granules. Upon cellular activation, it is deposited into pathogen-containing phagosomes.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
MPO
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Short name
Recombinant Myeloperoxidase/MPO Protein(C-10His)
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Human, Humans
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Alternative name
Rec. H. sapiens Myeloperoxidase/myeloperoxidase Protein(C-10His)
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Alternative technique
rec
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Alternative to gene target
myeloperoxidase, MPO and IDBG-60932 and ENSG00000005381 and 4353, metal ion binding, nuclei, Mpo and IDBG-207719 and ENSMUSG00000009350 and 17523, MPO and IDBG-629390 and ENSBTAG00000011616 and 511206
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Gene info
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Identity
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Gene
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Long gene name
myeloperoxidase
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Locus
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Discovery year
2001-06-22
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Entrez gene record
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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