Recombinant Human MAP1D / Methionine Aminopeptidase 1D Protein (His tag) 50µg

• Catalog number
  PKSH031376-50µg
• Price
  Please ask
• Size
  50µg

• Synonym
  MAP 1D;MAP1D;MetAP 1D;Metap1l
• Activity
  Measure by its ability to remove methionine from a fluorogenic peptide substrate H-Met-Gly-Pro-AMC, R&D Systems, Catalog#ES017. The resulting GP-AMC is cleaved by human DPPIV/CD26, R&D Systems, Catalog#1180SE. The specific activity is >30 pmoles/min/μg.
• Sequence
  Arg 44-Ala 335
• Fusion tag
  C-His
• Accession
  NP_954697.1
• Expressed Host
  Baculovirus-Insect Cells
• Shipping
  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
• Purity
  ＞92 % as determined by SDS-PAGE
• Endotoxin
  ＜1.0 EU per µg as determined by the LAL method
• Stability and Storage
  Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Mol Mass
  33.4 kDa
• AP Mol Mass
  36 kDa
• Formulation
  Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0
• Reconstitution
  Please refer to it for detailed information.
• Background
  Methionine aminopeptidase 1D, also known as MAP1D, is a member of the peptidase M24A family. N-terminal methionine removal is an important cellular process required for proper biological activity, subcellular localization, and eventual degradation of many proteins. The enzymes that catalyze this reaction are called Methionine aminopeptidases (MAPs). MAP1D is overexpressed in colon cancer cell lines and colon tumors as compared to normal tissues (at protein level). Downregulation of MAP1D expression by shRNA in HCT-116 colon carcinoma cells reduces anchorage-independant growth in soft agar. MAP1D binds two cobalt ions per subunit. The true nature of the physiological cofactor is under debate. MAP1D is also active with zinc, manganese or divalent ions. MAP1D removes the amino-terminal methionine from nascent proteins. It may also play an important role in colon tumorigenesis.
• Product URL
• PDF URL
• Conjugation
  histidine
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  MAP1D   Methionine   Aminopeptidase   Protein   His   tag   50µg  
• Gene symbol
  METAP1D
• Short name
  Recombinant MAP1D / Methionine Aminopeptidase 1D Protein (His tag) 50µg
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
• Label
  His
• Species
  Human, Humans
• Alternative name
  Rec. H. sapiens MAP1D / Methionine Aminopeptidase 1D Protein (histidine detection labelled) 50µg
• Alternative technique
  rec

Gene info

• Identity
  HGNC:32583
• Gene
  METAP1D
• Long gene name
  methionyl aminopeptidase type 1D, mitochondrial
• Synonyms
  • MAP1D
  • Metap1l
• Synonyms name
  • methionine aminopeptidase 1D
• GenBank acession
  • AY374142
  • BC029123
• Locus
  2q31.1
• Discovery year
  2010-09-21
• Entrez gene record
  254042
• Pubmed identfication
  • 14532271
  • 16568094
• RefSeq identity
  • NM_199227
• Classification
  • Aminopeptidases
• VEGA ID
  OTTHUMG00000132283

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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