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Synonym
Mannan-Binding Lectin Serine Protease 1; Complement Factor MASP-3;Mannose-Binding Lectin-Associated Serine Protease 1; MASP-1; RaRF; Serine Protease 5; MASP1; CRARF; CRARF1; PRSS5
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Activity
NA
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Sequence
His20-Arg728
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Fusion tag
C-6His
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Accession
P48740
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Expressed Host
Human cells
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Shipping
The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.
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Purity
＞95% as determined by reducing SDS-PAGE.
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Endotoxin
＜1.0 EU per µg as determined by LAL test.
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Stability and Storage
Store at < -20℃, stable for 6 monthshs after receipt.Please minimize freeze-thaw cycles
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Mol Mass
80.7 kDa
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AP Mol Mass
120 kDa
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Formulation
Supplied as a 0.2 μm filtered solution of 20mM Tris,200mM NaCl,10％Glycerol,pH8.0.
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Background
Mannan-Binding Lectin Serine Protease 1 (MASP-1) belongs to the peptidase S1 family. MASP1 contains two CUB domains, one EGF-like domain, one peptidase S1 domain and two Sushi (CCP/SCR) domains. MASP1 is primarily expressed in liver. MASP1 involved in the lectin pathway of the complement, performs a key role in innate immunity by recognizing pathogens through patterns of sugar moieties and neutralizing them. MASP1 is synthesized as a zymogen and activated when it complexes with the pathogen recognition molecules of lectin pathway, the mannose-binding lectin and the ficolins. MASP1 is not directly involved in complement activation but may act as an amplifier of complement activation by cleaving complement C2 or by activating another complement serine protease, MASP2. MASP1 is also able to cleave fibrinogen and factor XIII and may may be involved in coagulation. MASP1 is inhibited by SERPING1 and A2M.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Source
Recombinants or rec. proteins
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Group
recombinants
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Description
Serine protease, D- or L-serine arginine rich enzyme of serine threonine kinase with serine that is encoded by the codons UCU, UCC, UCA, UCG, AGU and AGC is an ɑ-amino acid that is used in the biosynthesis of proteins. It contains an α-amino group (which is in the protonated −NH+
3 form under biological conditions), a carboxyl group. It is non-essential in humans, meaning the body can synthesize it.
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Gene target
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Gene symbol
MASP1
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Short name
Recombinant Mannan-Binding Lectin Serine Protease 1/MASP1 Protein(C-6His)
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Human, Humans
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Alternative name
Rec. H. sapiens Mannan-Binding Lectin Serine Protease 1/mannan-binding lectin serine peptidase 1 (C4/complement component 2 activating component on Ra-reactive factor) Protein(C-6His)
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Alternative technique
rec
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Alternative to gene target
mannan-binding lectin serine peptidase 1 (C4/C2 activating component of Ra-reactive factor), 3MC1 and CRARF and CRARF1 and MAP1 and MAp44 and MASP and MASP3 and PRSS5 and RaRF, MASP1 and IDBG-69257 and ENSG00000127241 and 102725441,5648, calcium-dependent protein binding, Extracellular, Masp1 and IDBG-149706 and ENSMUSG00000022887 and 17174, MASP1 and IDBG-634626 and ENSBTAG00000012467 and 522347
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