Recombinant Human LTC4S / LTC4 synthase Protein (His tag) 50µg

• Catalog number
  PKSH031060-50µg
• Price
  Please ask
• Size
  50µg

• Synonym
  LTC4S;MGC33147
• Activity
  NA
• Sequence
  Met 1-Ala 150
• Fusion tag
  C-His
• Accession
  NP_665874.1
• Expressed Host
  Baculovirus-Insect Cells
• Shipping
  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
• Purity
  ＞90 % as determined by SDS-PAGE
• Endotoxin
  ＜1.0 EU per µg as determined by the LAL method
• Stability and Storage
  Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Mol Mass
  17 kDa
• AP Mol Mass
  17 kDa
• Formulation
  Lyophilized from sterile 50mM Hepes, 0.1% Triton 0.5% DOC, 10% Gly, pH 8.0
• Reconstitution
  Please refer to it for detailed information.
• Background
  Leukotriene C4 synthase, also known as LTC4 synthase, Leukotriene-C(4) synthase, and LTC4S, is a multi-pass membrane protein which belongs to the MAPEG family. LTC4S is detected in lung, platelets and the myelogenous leukemia cell line KG-1 (at protein level). LTC4S activity is present in eosinophils, basophils, mast cells, certain phagocytic mononuclear cells, endothelial cells, vascular smooth muscle cells and platelets. LTC4S is essential for the production of cysteinyl leukotrienes (Cys-LT), critical mediators in asthma. Mutagenic analysis of the conjugation function of human LTC4S has identified R51 and Y93 as critical for acid and base catalysis of LTA4 and reduced glutathione, respectively. A comparison across species for proteins that possess LTC4S activity reveals conservation of both of these residues, whereas R51 is absent in the FLAP molecules. Thus, within the glutathione S-transferase superfamily of genes, alignment of specific residues allows the separation of LTC4S family members from their most structurally similar counterparts, the FLAP molecules. Defects in LTC4S are the cause of leukotriene C4 synthase deficiency (LTC4 synthase deficiency). LTC4 synthase deficiency is a fatal neurometabolic developmental disorder. It is associated with muscular hypotonia, psychomotor retardation, failure to thrive, and microcephaly.
• Product URL
• PDF URL
• Conjugation
  histidine
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  LTC4S   LTC4   synthase   Protein   His   tag   50µg  
• Gene symbol
  LTC4S
• Short name
  Recombinant LTC4S / LTC4 synthase Protein (His tag) 50µg
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
• Label
  His
• Species
  Human, Humans
• Alternative name
  Rec. H. sapiens leukotriene C4 synthase / LTC4 synthase Protein (histidine detection labelled) 50µg
• Alternative technique
  rec
• Alternative to gene target
  leukotriene C4 synthase, LTC4S and IDBG-243666 and ENSG00000213316 and 4056, lipid binding, nuclei, Ltc4s and IDBG-168449 and ENSMUSG00000020377 and 17001, LTC4S and IDBG-640977 and ENSBTAG00000009737 and 511749

Gene info

• Identity
  HGNC:6719
• Gene
  LTC4S
• Long gene name
  leukotriene C4 synthase
• Synonyms
  • MGC33147
• GenBank acession
  • U11552
• Locus
  5q35.3
• Discovery year
  1994-11-24
• Entrez gene record
  4056
• Pubmed identfication
  • 8052639
• RefSeq identity
  • NM_000897
• VEGA ID
  OTTHUMG00000150314

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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