Recombinant Human KYNU / Kynureninase Protein (His tag)

• Catalog number
  PKSH031397-10µg
• Price
  Please ask
• Size
  10µg

• Synonym
  KYNUU
• Activity
  Measured by its ability to oxidize 3-hydroxykynurenine.The specific activity is > 200 pmoles/min/μg.
• Sequence
  NA
• Fusion tag
  NA
• Accession
  Q16719
• Expressed Host
  Baculovirus-Insect Cells
• Shipping
  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
• Purity
  > 92 % as determined by SDS-PAGE
• Endotoxin
  < 1.0 EU per μg of the protein as determined by the LAL method
• Stability and Storage
  Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Mol Mass
  53.7 kDa
• AP Mol Mass
  47 kDa
• Formulation
  Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0, 25% gly
• Background
  Protein disulfide-isomerase, also known as Cellular thyroid hormone-binding protein, Prolyl 4-hydroxylase subunit beta, p55 and P4HB, is a peripheral membrane protein which belongs to the protein disulfide isomerase family. P4HB is highly abundant. In some cell types, it seems to be also secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources. P4HB localizes near CD4-enriched regions on lymphoid cell surfaces. It is identified by mass spectrometry in melanosome fractions from stage I to stage IV. P4HB reduces and may activate fusogenic properties of HIV-1 gp120 surface protein, thereby enabling HIV-1 entry into the cell. P4HB catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, it seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. P4HB may therefore cause structural modifications of exofacial proteins. Inside the cell, it seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, P4HB functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, it facilitates aggregation (anti-chaperone activity). P4HB may be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. It also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP.
• Technical data
  #N/A
• Conjugation
  histidine
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  KYNU   Kynureninase   Protein   His   tag  
• Gene symbol
  KYNU
• Short name
  Recombinant KYNU / Kynureninase Protein (His tag)
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
• Label
  His
• Species
  Human, Humans
• Alternative name
  Rec. H. sapiens kynureninase / Kynureninase Protein (histidine detection labelled)
• Alternative technique
  rec
• Alternative to gene target
  kynureninase, KYNU and IDBG-71532 and ENSG00000115919 and 8942, protein homodimerization activity, Cytoplasm, Kynu and IDBG-167886 and ENSMUSG00000026866 and 70789, BT.79081 and IDBG-642133 and ENSBTAG00000032277 and 520327

Gene info

• Identity
  HGNC:6469
• Gene
  KYNU
• Long gene name
  kynureninase
• Synonyms gene name
  • kynureninase (L-kynurenine hydrolase)
• Synonyms name
  • L-kynurenine hydrolase
• GenBank acession
  • U57721
• Locus
  2q22.2
• Discovery year
  1999-09-20
• Entrez gene record
  8942
• Pubmed identfication
  • 8706755
  • 9180257
  • 28792876
• RefSeq identity
  • NM_001032998
• VEGA ID
  OTTHUMG00000131829

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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