Recombinant Human ITCH / AIP4 Protein (aa 526-903)

• Catalog number
  PKSH031249-20µg
• Price
  Please ask
• Size
  20µg

• Synonym
  ADMFD;AIF4;AIP4;dJ468O1.1;NAPP1
• Activity
  NA
• Sequence
  Arg 526-Glu 903
• Fusion tag
  NA
• Accession
  NP_113671.3
• Expressed Host
  E. coli
• Shipping
  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
• Purity
  ＞98 % as determined by SDS-PAGE
• Endotoxin
  Please contact us for more information.
• Stability and Storage
  Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Mol Mass
  40 kDa
• AP Mol Mass
  40 kDa
• Formulation
  Lyophilized from sterile 20mM Tris, 200mM NaCl, 10% glycerol, pH 8.0
• Background
  E3 ubiquitin-protein ligase Itchy homolog, also known as Atrophin-1-interacting protein 4, NFE2-associated polypeptide 1, NAPP1 and ITCH, is a cell membrane protein which contains one C2 domain, one HECT (E6AP-type E3 ubiquitin-protein ligase) domain and contains four WW domains. ITCH acts as an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. It catalyzes 'Lys-29'-, 'Lys-48'- and 'Lys-63'-linked ubiquitin conjugation. ITCH is involved in the control of inflammatory signaling pathways. It is an essential component of a ubiquitin-editing protein complex, comprising also TNFAIP3, TAX1BP1 and RNF11, that ensures the transient nature of inflammatory signaling pathways. ITCH promotes the association of the complex after TNF stimulation. Once the complex is formed, TNFAIP3 deubiquitinates 'Lys-63' polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteosomal degradation and consequently termination of the TNF- or LPS-mediated activation of NFKB1. Defects in ITCH are the cause of syndromic multisystem autoimmune disease (SMAD) which is characterized by organomegaly, failure to thrive, developmental delay, dysmorphic features and autoimmune inflammatory cell infiltration of the lungs, liver and gut.
• Technical data
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  ITCH   AIP4   Protein   526-903  
• Gene symbol
  ITCH, ITCH-AS1, ITCH-IT1
• Short name
  Recombinant ITCH / AIP4 Protein (aa 526-903)
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  Rec. H. sapiens itchy E3 ubiquitin protein ligase / AIP4 Protein (aa 526-903)
• Alternative technique
  rec
• Alternative to gene target
  itchy E3 ubiquitin protein ligase, ITCH and IDBG-68143 and ENSG00000078747 and 83737, CXCR chemokine receptor binding, nuclei, Itch and IDBG-210517 and ENSMUSG00000027598 and 16396, ITCH and IDBG-641890 and ENSBTAG00000000308 and 508431

Gene info

• Identity
  HGNC:13890
• Gene
  ITCH
• Long gene name
  itchy E3 ubiquitin protein ligase
• Synonyms gene name
  • itchy (mouse homolog) E3 ubiquitin protein ligase
  • itchy E3 ubiquitin protein ligase homolog (mouse)
• Synonyms
  • AIP4
• GenBank acession
  • AF095745
• Locus
  20q11.22
• Discovery year
  2001-04-27
• Entrez gene record
  83737
• Pubmed identfication
  • 11318614
• Classification
  • C2 domain containing
  • HECT domain containing
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000032300
• Locus Specific Databases
  • LRG_354

Gene info

• Identity
  HGNC:40659
• Gene
  ITCH-AS1
• Long gene name
  ITCH antisense RNA 1
• Synonyms gene name
  • ITCH antisense RNA 1 (non-protein coding)
• Locus
  20q11.22
• Discovery year
  2011-05-19
• Classification
  • Antisense RNAs
• VEGA ID
  OTTHUMG00000032298

Gene info

• Identity
  HGNC:41397
• Gene
  ITCH-IT1
• Long gene name
  ITCH intronic transcript 1
• Synonyms gene name
  • ITCH intronic transcript 1 (non-protein coding)
• Locus
  20q11.22
• Discovery year
  2011-05-19
• Classification
  • Intronic transcripts
• VEGA ID
  OTTHUMG00000046196

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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