Recombinant Human IL7RA / CD127 Protein (His & Fc tag)

• Catalog number
  PKSH031328-50µg
• Price
  Please ask
• Size
  50µg

• Synonym
  CD127;CDW127;IL-7R;IL-7R-alpha;IL7RA;ILRA
• Activity
  Measured by its ability to bind biotinylated mouse TSLP-his in functional ELISA.2. Measured by its binding ability in a functional ELISA. Immobilized human IL7 at 10 μg/ml (100 μl/well) can bind human IL7Ra-Fch, The EC50 of human IL7Ra-Fch is 15.2-35.6 ng/ml.
• Sequence
  Met 1-Gly 236
• Fusion tag
  C-His & Fc
• Accession
  NP_002176.2
• Expressed Host
  HEK293 Cells
• Shipping
  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
• Purity
  ＞95 % as determined by SDS-PAGE
• Endotoxin
  ＜1.0 EU per µg as determined by the LAL method
• Stability and Storage
  Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Mol Mass
  53 kDa
• AP Mol Mass
  65-75 kDa
• Formulation
  Lyophilized from sterile PBS, pH 7.4
• Background
  Interleukin 7 Receptor alpha (IL-7RA), also known as CD127, is a 75 kDa hematopoietin receptor superfamily member that plays an important role in lymphocyte differentiation, proliferation, and survival. IL-7 receptor alpha (CD127) signaling is essential for T-cell development and regulation of naive and memory T-cell homeostasis. IL-7RA is critically required for the proper development and function of lymphoid cells. Therefore, the IL-7RA is critically required for the proper development and function of lymphoid cells. Studies from both pathogenic and controlled HIV infection indicate that the containment of immune activation and preservation of CD127 expression are critical to the stability of CD4(+) T cells in infection. A better understanding of the factors regulating CD127 expression in HIV disease, particularly on T(CM) cells, might unveil new approaches exploiting the IL-7/IL-7R receptor pathway to restore T cell homeostasis and promote immune reconstitution in HIV infection. Factors relevant to HIV infection that could potentially decrease CD127 expression on human CD8(+) T cells. CD127 down-regulation may be an important contributor to HIV-associated T-cell dysfunction. In addition to IL-7, IL-7RA also associates with TSLPR to form the functional receptor for thymic stromal lymphopoietin (TSLP) which indirectly regulates T cell development by modulating dendritic cell activation. Mutations in the human IL-7RA gene cause a type of severe combined immunodeficiency in which the major deficiencies are in T cell development, whereas B and NK cells are relatively normal in number. Variation in the IL7RA gene was recently found associated with multiple sclerosis (MS). The polymorphisms in the IL7RA gene is involved in MS pathogenesis and suggest that IL7RA variation may primarily affect chronic disease courses. Soluble CD127 (sCD127) appears to play an important role in the immunopathogenesis of several chronic infections, multiple sclerosis, and various cancers.
• Technical data
• Conjugation
  histidine
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  IL7RA   CD127   Protein   His   tag  
• Gene symbol
  IL7R
• Short name
  Recombinant IL7RA / CD127 Protein (His & Fc tag)
• Technique
  Recombinant, FC, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
• Label
  His
• Species
  Human, Humans
• Alternative name
  Rec. H. sapiens IL7RA / CD127 Protein (histidine & fragment c detection labelled)
• Alternative technique
  rec

Gene info

• Identity
  HGNC:6024
• Gene
  IL7R
• Long gene name
  interleukin 7 receptor
• Synonyms
  • CD127
  • IL7RA
• GenBank acession
  • M29696
• Locus
  5p13.2
• Discovery year
  1991-08-07
• Entrez gene record
  3575
• Pubmed identfication
  • 2317865
• Classification
  • Interleukin receptors
  • Fibronectin type III domain containing
  • CD molecules
• VEGA ID
  OTTHUMG00000090791
• Locus Specific Databases
  • IL7Rbase: Mutation registry for Interleukin-7 receptor α deficiency
  • LRG_74

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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