Recombinant Human IDO1 / IDO Protein
-
Catalog number
PKSH030355-10µg
-
Price
Please ask
-
Size
10µg
-
-
Synonym
IDO;IDO-1;INDO;Indoleamine 2,3‑dioxygenase
-
Activity
Measured by its ability to oxidize L-tryptophan to N-formylk-ynurenine.The specific activity is > 500 pmoles/min/μg.
-
Sequence
Ala2-Gly403
-
Fusion tag
NA
-
Accession
NP_002155.1
-
Expressed Host
E. coli
-
Shipping
Liquid. It is shipped out with blue ice.
-
Purity
＞85 % as determined by SDS-PAGE
-
Endotoxin
Please contact us for more information.
-
Stability and Storage
Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Mol Mass
45.2 kDa
-
AP Mol Mass
46 kDa
-
Formulation
Lyophilized from sterile 50 mM NaAC, 100 mM Nacl, 20 % glycerol, pH 5.5
-
Background
Indoleamine 2,3-dioxygenase-1, also known as Indoleamine-pyrrole 2,3-dioxygenase, IDO1 and IDO, is a member of the indoleamine 2,3-dioxygenase family. IDO1 / IDO and tryptophan 2,3-dioxygenase (TDO) are tryptophan-degrading enzymes that catalyze the first step in tryptophan catabolism via the kynurenine pathway. TDO is widely distributed in both eukaryotes and bacteria. In contrast, IDO has been found only in mammals and yeast. In 2007, a third enzyme, indoleamine 2,3-dioxygenase-2 (IDO2), was discovered. IDO2 is found not only in mammals but also in lower vertebrates. IDO1 / IDO is an immunosuppressive molecule inducible in various cells. IDO1 / IDO catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen. It mediates oxidative cleavage of tryptophan, an amino acid essential for cell proliferation and survival. IDO1 / IDO inhibition is proposed to have therapeutic potential in immunodeficiency-associated abnormalities, including cancer. The IDO pathway is activated in multiple tumor types. Selective inhibition of IDO1 may represent an attractive cancer therapeutic strategy via up-regulation of cellular immunity. IDO1 / IDO is an enzyme that suppresses adaptive T-cell immunity by catabolizing tryptophan from the cellular microenvironment. Inhibition of IDO pathway might enhance the efficacy of immunotherapeutic strategies for cancer.Immune CheckpointImmune Checkpoint Detection: ELISA AntibodiesCo-inhibitory Immune Checkpoint Targets Immunotherapy Cancer Immunotherapy Targeted Therapy
-
-
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
Source
Recombinants or rec. proteins
-
Group
recombinants
-
Gene target
-
Gene symbol
IDO1
-
Short name
Recombinant IDO1 / IDO Protein
-
Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
-
Species
Human, Humans
-
Alternative name
Rec. H. sapiens indoleamine 2,3-dioxygenase 1 / Indoleamine 2,3-dioxygenase 1 Protein
-
Alternative technique
rec
-
Alternative to gene target
indoleamine 2,3-dioxygenase 1, IDO and IDO-1 and INDO, IDO1 and IDBG-18750 and ENSG00000131203 and 3620, tryptophan 2, Cytoplasm, Ido1 and IDBG-142448 and ENSMUSG00000031551 and 15930, BT.19792 and IDBG-629572 and ENSBTAG00000020602 and 506281
-
Gene info
MeSH Data
-
Name
-
Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
-
Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
Similar products
