Recombinant Human Histone cluster 2 H2BE / HIST2H2BE Protein

• Catalog number
  PKSH031141-20µg
• Price
  Please ask
• Size
  20µg

• Synonym
  GL105;H2B;H2B.1;H2BFQ;H2BGL105;H2BQ;MGC119802;MGC119804;MGC129733;MGC129734
• Activity
  NA
• Sequence
  Met 1-Lys 126
• Fusion tag
  NA
• Accession
  Q16778
• Expressed Host
  E. coli
• Shipping
  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
• Purity
  ＞95 % as determined by SDS-PAGE
• Endotoxin
  Please contact us for more information.
• Stability and Storage
  Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Mol Mass
  14.2 kDa
• AP Mol Mass
  16 kDa
• Formulation
  Lyophilized from sterile 2mM β-Mercaptoethanol, pH 6.9
• Background
  Histones are a complex family of highly conserved basic proteins responsible for packaging chromosomal DNA into nucleosomes. Histone proteins exhibit two levels of diversity: 1. evolutionary diversity between species and 2. subtype diversity in a class(H1, H2A, H2B, H3 or H4) within a species. It has become more and more evident that histone modifications are key players in the regulation of chromatin states and dynamics as well as in gene expression. Therefore, histone modifications and the enzymatic machineries that set them are crucial regulators that can control cellular proliferation, differentiation, plasticity, and malignancy processes. However, extracellular histones are a double-edged sword because they also damage host tissue and may cause death. Histones bound to platelets, induced calcium influx, and recruited plasma adhesion proteins such as fibrinogen to induce platelet aggregation. Histone H2B proteins have been studied in a variety of species and is easily detecred in most species. The reversible ubiquitylation of histone H2B has long been implicated in transcriptional activation and gene silencing. Phosphorylation of H2B serine 32 occurs in normal cycling and mitogen-stimulated cells. Notably, this phosphorylation is elevated in skin cancer cell lines and tissues compared with normal counterparts. HIST2H2BE is a member of the histone H2B family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.
• Technical data
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Histone   cluster   H2BE   HIST2H2BE   Protein  
• Gene symbol
  H2BC21
• Short name
  Recombinant Histone cluster 2 H2BE / HIST2H2BE Protein
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  Rec. H. sapiens Histone cluster 2 H2BE / HIST2H2BE Protein
• Alternative technique
  rec

Gene info

• Identity
  HGNC:4760
• Gene
  H2BC21
• Long gene name
  H2B clustered histone 21
• Synonyms gene
  • H2B
  • H2BFQ
  • HIST2H2BE
• Synonyms gene name
  • H2B histone family, member Q
  • histone 2, H2be
  • histone cluster 2, H2be
  • histone cluster 2 H2B family member e
• Synonyms
  • H2B/q
  • H2B.1
  • H2BE
  • H2B-GL105
• GenBank acession
  • AY131979
• Locus
  1q21.2
• Discovery year
  1998-08-27
• Entrez gene record
  8349
• Pubmed identfication
  • 1469070
  • 12408966
  • 23240083
• RefSeq identity
  • NM_003528
• Classification
  • H2B histones
• VEGA ID
  OTTHUMG00000012095

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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