Recombinant Human Her2 / ERBB2 Protein (His Tag)
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Catalog number
PKSH031997-50µg
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Price
Please ask
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Size
50µg
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Synonym
CD340;ENV;ENVW;ERVWE1;HER-2;HER-2/neu;HER2;HERV-7q;HERV-W-ENV;HERV7Q;HERVW;HERVWENV;MLN 19;MLN19;NEU;NGL;TKR1
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Activity
Measured by its binding ability in a functional ELISA. Immobilized human Erbb2 at 2.5 μg/ml (100 μl/well) can bind Herceptin with a linear ranger of 1.28-32 ng/ml.
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Sequence
Met 1-Thr 652
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Fusion tag
C-His
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Accession
NP_004439.2
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Expressed Host
HEK293 Cells
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Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
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Purity
>90 % as determined by SDS-PAGE
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Endotoxin
<1.0 EU per µg as determined by the LAL method
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Stability and Storage
Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Mol Mass
71 kDa
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AP Mol Mass
100-110 kDa
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Formulation
Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. 2. Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA.
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Background
Epidermal growth factor receptor 2 (HER2), also known as ErbB2, NEU, and CD340, is a type I membrane glycoprotein, and belongs to the epidermal growth factor (EGF) receptor family. HER2 protein cannot bind growth factors due to the lacking of ligand binding domain of its own and autoinhibited constitutively. However, HER2 forms a heterodimer with other ligand-bound EGF receptor family members, therefore stabilizes ligand binding and enhances kinase-mediated activation of downstream molecules. HER2 plays a key role in development, cell proliferation and differentiation. HER2 gene has been reported to associate with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate, ovarian, lung cancers and so on.Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy
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Conjugation
histidine
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
ERBB2
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Short name
Recombinant Her2 / ERBB2 Protein (His Tag)
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
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Label
His
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Species
Human, Humans
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Alternative name
Rec. H. sapiens Her2 / v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) Protein (histidine detection labelled)
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Alternative technique
rec
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Alternative to gene target
v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian), CD340 and HER-2 and HER-2/neu and HER2 and MLN 19 and NEU and NGL and TKR1, ERBB2 and IDBG-46068 and ENSG00000141736 and 2064, transferase activity, nuclei, Erbb2 and IDBG-210591 and ENSMUSG00000062312 and 13866, ERBB2 and IDBG-639410 and ENSBTAG00000021798 and 505709
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Gene info
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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