Recombinant Human FAS / CD95 / APO-1 / TNFRSF6 Protein (Fc tag) 100µg
-
Catalog number
PKSH031751-100µg
-
Price
Please ask
-
Size
100µg
-
-
Synonym
ALPS1A;APO-1;APT1;CD95;FAS1;FASTM;TNFRSF6
-
Activity
Measured by its ability to inhibit Fas Ligand induced apoptosis of Jurkat human acute T cell leukemia cells.The ED50 for this effect is typically 0.02-0.1 μg/mL in the presence of 20 ng/mL recombinant human Fas ligand.
-
Sequence
Met 1-Glu 173
-
Fusion tag
C-Fc
-
Accession
NP_000034.1
-
Expressed Host
HEK293 Cells
-
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
-
Purity
(75.9+20.9) % as determined by SDS-PAGE
-
Endotoxin
＜1.0 EU per µg as determined by the LAL method
-
Stability and Storage
Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Mol Mass
43.4 kDa
-
AP Mol Mass
55-60 kDa
-
Formulation
Lyophilized from sterile PBS, pH 7.4
-
Reconstitution
Please refer to it for detailed information.
-
Background
CD95 (APO-1/Fas) is an important inducer of the extrinsic apoptosis signaling pathway and therapy induced apoptosis of many tumor cells has been linked to the activity of CD95. is a prototype death receptor characterized by the presence of an 80 amino acid death domain in its cytoplasmic tail. This domain is essential for the recruitment of a number of signaling components upon activation by either agonistic anti-CD95 antibodies or cognate CD95 ligand that initiate apoptosis. The complex of proteins that forms upon triggering of CD95 is called the death-inducting signaling complex (DISC). The DISC consists of an adaptor protein and initiator caspases and is essential for induction of apoptosis. CD95 is also crucial for the negative selection of B cells within the germinal center (GC). Impairment of CD95-mediated apoptosis results in defective affinity maturation and the persistence of autoreactive B-cell clones. Changes in the expression of CD95 and/or its ligand CD95L are frequently found in human cancer. The downregulation or mutation of CD95 has been proposed as a mechanism by which cancer cells avoid destruction by the immune system through reduced apoptosis sensitivity. Thus, CD95 has therefore been viewed as a tumor suppressor. CD95 has been reported to be involved in the activation of NF-kappaB, MAPK3/ERK1, MAPK8/JNK, and the alternate pathways for CTL-mediated cytotoxicity. Accordingly, this protein is implicated in the pathogenesis of various malignancies and diseases of the immune system. The CD95/CD95L system was implicated in the etiology of inflammatory bowel disease (IBD) based, primarily, on the finding that CD95 is highly expressed in the intestinal epithelial cells and that epithelial apoptosis is increased in IBD.
-
-
-
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
Source
Recombinants or rec. proteins
-
Group
recombinants
-
Gene target
-
Gene symbol
FAS, FAS-AS1, FASN, TNFSF10, AOPEP, TNFRSF25, IGHVII-1-1, MIR1-1, TRUND-NNN8-1, TRUND-NNN7-1
-
Short name
Recombinant FAS / CD95 / APO-1 / TNFRSF6 Protein (Fc tag) 100µg
-
Technique
Recombinant, FC, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
-
Label
Fc Tag
-
Species
Human, Humans
-
Alternative name
Rec. H. sapiens Fas (tumor necrosis factor receptor superfamily, member 6) / CD95 / APO-1 / TNFRSF6 Protein (fragment c detection labelled) 100µg
-
Alternative technique
rec
-
Alternative to gene target
Fas (TNF receptor superfamily, member 6), ALPS1A and APO-1 and APT1 and CD95 and FAS1 and FASTM and TNFRSF6, FAS and IDBG-81669 and ENSG00000026103 and 355, identical protein binding, nuclei, Fas and IDBG-159577 and ENSMUSG00000024778 and 14102, FAS and IDBG-637356 and ENSBTAG00000010785 and 282488
-
Gene info
Gene info
-
Identity
-
Gene
-
Long gene name
FAS antisense RNA 1
-
Synonyms gene
-
Synonyms gene name
- FAS antisense RNA (non-protein coding)
- FAS antisense RNA 1 (non-protein coding)
-
Synonyms
-
Locus
-
Discovery year
2009-07-16
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
Gene info
-
Identity
-
Gene
-
Long gene name
fatty acid synthase
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year
1994-05-17
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Short chain dehydrogenase/reductase superfamily
- 7BS orphan methyltransferases
-
VEGA ID
Gene info
Gene info
Gene info
Gene info
Gene info
-
Identity
-
Gene
-
Long gene name
microRNA 1-1
-
Synonyms gene
-
Synonyms
-
Locus
-
Discovery year
2004-04-23
-
Entrez gene record
-
RefSeq identity
-
Classification
Gene info
Gene info
MeSH Data
-
Name
-
Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
-
Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
Similar products
