-
Synonym
Ephrin type-A receptor 4;HEK8; SEK; TYRO1;EPHA4;Tyrosine-protein kinase receptor SEK;Tyrosine-protein kinase TYRO1;EK8;hEK8;EPH-like kinase 8
-
Activity
The specific activity was determined to be 17 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substrate.2. Measured by its binding ability in a functional ELISA. Immobilized human EPHA4 (aa 570-986)at 10 μg/ml (100 μl/well) can bind biotinylated human EphrinA5-His with a linear range of 0.625-5.0 μg/ml.
-
Sequence
Val20-Thr547
-
Fusion tag
NA
-
Accession
P54764
-
Expressed Host
Human cells
-
Shipping
The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.
-
Purity
＞95% as determined by reducing SDS-PAGE.
-
Endotoxin
＜1.0 EU per µg as determined by LAL test.
-
Stability and Storage
Lyophilized protein should be stored at < -20℃, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7℃ for 2-7 days.Aliquots of reconstituted samples are stable at < -20℃ for 3 months
-
Mol Mass
59.2 kDa
-
AP Mol Mass
59.2 kDa
-
Formulation
Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
-
Reconstitution
Please refer to it for detailed information.
-
Background
Ephrin type-A receptor 4(EPHA4) belongs to the protein kinase superfamily and Ephrin receptor subfamily. EPHA4 contains 1 Eph LBD domain, 2 fibronectin type-III domains, 1 protein kinase domain and 1 SAM domain. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands.
-
-
-
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
Description
The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
-
Source
Recombinants or rec. proteins
-
Group
recombinants
