Recombinant Human Cystatin A / CSTA Protein (His tag)

• Catalog number
  PKSH031581-50µg
• Price
  Please ask
• Size
  50µg

• Synonym
  AREI;STF1;STFA
• Activity
  NA
• Sequence
  Ile 2-Phe 98
• Fusion tag
  N-His
• Accession
  P01040
• Expressed Host
  E. coli
• Shipping
  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
• Purity
  ＞95 % as determined by SDS-PAGE
• Endotoxin
  Please contact us for more information.
• Stability and Storage
  Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Mol Mass
  12.4 kDa
• AP Mol Mass
  14 kDa
• Formulation
  Lyophilized from sterile 50mM Tris, 1mM EDTA, pH 7.5
• Background
  Cystatin-A, also known as Cystatin-AS, Stefin-A and CSTA, is a cytoplasm protein which belongs to the cystatin family. Cystatin-A / CSTA is a cysteine proteinase inhibitor with a molecular mass of 11 kDa, and is located mainly in the keratohyaline granules of the stratum granulosum and the cornified envelope of the stratum corneum in the epidermis. The cystatins are a family of cysteine protease inhibitors with homology to chicken cystatin. Cystatins are physiological inhibitors of cysteine proteinases which are widely distributed in human tissues and fluids. Cystatins typically comprise about 115 amino acids, are largely acidic, contain four conserved cysteine residues known to form two disulfide bonds. Cystatins may be glycosylated and / or phosphorylated, with similarity to fetuins, kininogens, stefins, histidine-rich glycoproteins and cystatin-related proteins. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired inhibitory activity. Cystatins mainly inhibit peptidases belonging to peptidase families C1 (papain family) and C13 (legumain family).
• Technical data
• Conjugation
  histidine
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Cystatin   CSTA   Protein   His   tag  
• Gene symbol
  CSTA
• Short name
  Recombinant Cystatin A / CSTA Protein (His tag)
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
• Label
  His
• Species
  Human, Humans
• Alternative name
  Rec. H. sapiens Cystatin A / CSTA Protein (histidine detection labelled)
• Alternative technique
  rec

Gene info

• Identity
  HGNC:2481
• Gene
  CSTA
• Long gene name
  cystatin A
• Synonyms gene
  • STF1
  • STFA
• Synonyms gene name
  • cystatin A (stefin A)
• Synonyms name
  • stefin A
• Locus
  3q21.1
• Discovery year
  1990-02-06
• Entrez gene record
  1475
• Pubmed identfication
  • 1674139
• RefSeq identity
  • NM_005213
• Classification
  • Cystatins, type 1
• VEGA ID
  OTTHUMG00000159488

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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