Recombinant Human CHK1 / CHEK1 Protein (GST tag)
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Catalog number
PKSH030410-20µg
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Price
Please ask
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Size
20µg
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Synonym
CHK1
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Activity
The specific activity was determined to be 32 nmol/min/mg using CHKtide peptide (KKKVSRSGLYRSPSMPENLNRPR) as substrate.
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Sequence
Met1-Thr476
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Fusion tag
N-GST
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Accession
AAM78553.1
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Expressed Host
Baculovirus-Insect Cells
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Shipping
Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.Shipment made at ambient temperature may seriously affect the activity of the ordered products.
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Purity
＞90 % as determined by SDS-PAGE
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Endotoxin
＜1.0 EU per µg as determined by the LAL method
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Stability and Storage
Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Mol Mass
80.7kDa
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AP Mol Mass
80.7 kDa
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Formulation
Supplied as sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol, 2mM GSH
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Background
CHK1 / CHEK1 contains 1 protein kinase domain and belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family, NIM1 subfamily. It is a member of checkpoint kinases (Chks). Chks Checkpoint kinases (Chks) are serine/threonine kinases that are involved in the control of the cell cycle. There are two subtypes of chks that have so far been identified, CHK1 / CHEK1 and Chk2. They are essential components to delay cell cycle progression in normal and damaged cells and can act at all three cell cycle checkpoints. Chks are activated by phosphorylation. ATR kinase phosphorylates CHK1 / CHEK1 in response to single strand DNA breaks and ATM kinase phosphorylates Chk2 in response to double strand breaks. Chks phosphorylate Cdc25 phosphatase at Ser216, which leads to Cdc25 sequestration in the cytoplasm. Chks have a role in the physiological stress of hypoxia/reoxygenation. CHK1 / CHEK1 is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. CHK1 / CHEK1 may also negatively regulate cell cycle progression during unperturbed cell cycles.Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
CHEK1, MGST3
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Short name
Recombinant CHK1 / CHEK1 Protein (GST tag)
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
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Label
tag
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Species
Human, Humans
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Alternative name
Rec. H. sapiens CHK1 / CHEK1 Protein (GST detection labelled)
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Alternative technique
rec
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Gene info
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Identity
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Gene
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Long gene name
checkpoint kinase 1
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Synonyms gene name
- CHK1 (checkpoint, S.pombe) homolog
- CHK1 checkpoint homolog (S. pombe)
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Synonyms
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GenBank acession
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Locus
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Discovery year
1998-04-21
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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