Recombinant Human Carbonic Anhydrase XII / CA12 Protein (His tag)

• Catalog number
  PKSH031505-10µg
• Price
  Please ask
• Size
  10µg

• Synonym
  CA12;CAXII;FLJ20151;HsT18816
• Activity
  Measured by its esterase activity. The specific activity is >40 pmoles/min/μg, as measured with 1 mM 4-Nitrophenyl acetate and 2.5 μg enzyme at 400 nm in 100 μL of 12.5 mM Tris, 75 mM NaCl, pH 7.5.
• Sequence
  Met 1-Gln 291
• Fusion tag
  C-His
• Accession
  NP_001209.1
• Expressed Host
  HEK293 Cells
• Shipping
  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
• Purity
  ＞97 % as determined by SDS-PAGE
• Endotoxin
  ＜1.0 EU per µg as determined by the LAL method
• Stability and Storage
  Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Mol Mass
  31.6 kDa
• AP Mol Mass
  40-45 kDa
• Formulation
  Lyophilized from sterile PBS, pH 7.4
• Background
  Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. CA12, also known as Car12 and carbonic anhydrase XII, is a type I  membrane enzyme of an N-terminal extracellular catalytic domain, a membrane-spanning α-helix, and a small intracellular C-terminal domain. It is highly expressed in colon, kidney, prostate, intestine and activated lymphocytes and moderately expressed in pancreas, ovary, and testis. Overexpression of the CA12 is observed in certain human cancers and is used as a tumor marker. rmCA12 corresponds to the extracellular domain and has both carbonic anhydrase activity and esterase activity.
• Technical data
• Conjugation
  histidine
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Carbonic   Anhydrase   XII   CA12   Protein   His   tag  
• Gene symbol
  CA12, SIGLEC12
• Short name
  Recombinant Carbonic Anhydrase XII / CA12 Protein (His tag)
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
• Label
  His
• Species
  Human, Humans
• Alternative name
  Rec. H. sapiens Carbonic Anhydrase XII / CA12 Protein (histidine detection labelled)
• Alternative technique
  rec

Gene info

• Identity
  HGNC:1371
• Gene
  CA12
• Long gene name
  carbonic anhydrase 12
• Synonyms gene name
  • carbonic anhydrase XII
• Synonyms
  • HsT18816
• GenBank acession
  • AF051882
• Locus
  15q22.2
• Discovery year
  1998-07-17
• Entrez gene record
  771
• Pubmed identfication
  • 9636197
• RefSeq identity
  • NM_001218
• Classification
  • Carbonic anhydrases
• VEGA ID
  OTTHUMG00000132881

Gene info

• Identity
  HGNC:15482
• Gene
  SIGLEC12
• Long gene name
  sialic acid binding Ig like lectin 12
• Synonyms gene
  • SIGLECL1
• Synonyms gene name
  • SIGLEC-like 1
  • sialic acid binding Ig-like lectin 12
  • sialic acid binding Ig-like lectin 12 (gene/pseudogene)
  • sialic acid binding Ig like lectin 12 (gene/pseudogene)
• Synonyms
  • SLG
  • S2V
  • Siglec-XII
  • Siglec-12
  • Siglec-L1
• GenBank acession
  • AF282256
• Locus
  19q13.41
• Discovery year
  2001-08-28
• Entrez gene record
  89858
• Pubmed identfication
  • 11409877
  • 11328818
  • 21555517
• RefSeq identity
  • NM_053003
• Classification
  • C2-set domain containing
  • I-set domain containing
  • Sialic acid binding Ig like lectins
  • V-set domain containing
• VEGA ID
  OTTHUMG00000165524

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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