Recombinant Human Carbonic Anhydrase IV / CA4 Protein (His tag) 20µg

• Catalog number
  PKSH031585-20µg
• Price
  Please ask
• Size
  20µg

• Synonym
  CAIV;Car4;RP17
• Activity
  Measured by its esterase activity. The specific activity is >2 pmoles/min/μg.
• Sequence
  Met1-Lys283
• Fusion tag
  C-His
• Accession
  NP_000708.1
• Expressed Host
  HEK293 Cells
• Shipping
  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
• Purity
  ＞96 % as determined by SDS-PAGE
• Endotoxin
  ＜1.0 EU per µg as determined by the LAL method
• Stability and Storage
  Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Mol Mass
  31.7 kDa
• AP Mol Mass
  30 kDa
• Formulation
  Lyophilized from sterile PBS, pH 7.4
• Reconstitution
  Please refer to it for detailed information.
• Background
  The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase IV (CAIV) is a membrane-associated enzyme anchored to plasma membrane surfaces by a phosphatidylinositol glycan linkage. CAIV is a high-activity isozyme in CO2 hydration comparable to that of CAII. Furthermore, CAIV is more active in HCO3- dehydration than is CAII. However, the esterase activity of CAIV is decreased 150-fold compared to CAII.
• Product URL
• PDF URL
• Conjugation
  histidine
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Carbonic   Anhydrase   CA4   Protein   His   tag   20µg  
• Gene symbol
  CA4
• Short name
  Recombinant Carbonic Anhydrase IV / CA4 Protein (His tag) 20µg
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
• Label
  His
• Species
  Human, Humans
• Alternative name
  Rec. H. sapiens Carbonic Anhydrase IV / CA4 Protein (histidine detection labelled) 20µg
• Alternative technique
  rec

Gene info

• Identity
  HGNC:1375
• Gene
  CA4
• Long gene name
  carbonic anhydrase 4
• Synonyms gene
  • RP17
• Synonyms gene name
  • retinitis pigmentosa 17 (autosomal dominant)
  • carbonic anhydrase IV
• Synonyms
  • CAIV
  • Car4
• GenBank acession
  • L10955
• Locus
  17q23.1
• Discovery year
  1992-04-07
• Entrez gene record
  762
• Pubmed identfication
  • 8325641
• RefSeq identity
  • NM_000717
• Classification
  • Carbonic anhydrases
• VEGA ID
  OTTHUMG00000179987

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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