Recombinant Human Carbonic Anhydrase III / CA3 Protein (His tag)
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Catalog numberPKSH031566-50µg
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PricePlease ask
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Size50µg
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SynonymCAIII;Car3
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ActivityMeasured by its esterase activity.The specific activity is >5 pmoles/min/μg.
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SequenceMet 1-Lys 260
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Fusion tagC-His
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AccessionNP_005172.1
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Expressed HostE. coli
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ShippingIn general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
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Purity>95 % as determined by SDS-PAGE
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EndotoxinPlease contact us for more information.
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Stability and StorageSamples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Mol Mass30.4 kDa
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AP Mol Mass30.4 kDa
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FormulationLyophilized from sterile 50mM Tris, 500mM NaCl, 10% glycerol, pH 8.0
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BackgroundCarbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. Carbonic anhydrases (CAs) form a family of enzymes that catalyze the rapid conversion of carbon dioxide and water to bicarbonate and protons, a reaction that occurs rather slowly in the absence of a catalyst. The active site of most carbonic anhydrases contains a zinc ion, they are therefore classified as metalloenzymes. Several forms of carbonic anhydrase occur in nature. The primary function of the enzyme in animals is to interconvert carbon dioxide and bicarbonate to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide out of tissues. Plants contain a different form called β-carbonic anhydrase, which, from an evolutionary standpoint, is a distinct enzyme, but participates in the same reaction and also uses a zinc ion in its active site. Carbonic anhydrase 3, also known as Carbonate dehydratase III, CA-III and CA3, is a cytoplasm protein which belongs to the alpha-carbonic anhydrase family. CA3 is activated by proton donors such as imidazole and the dipeptide histidylhistidine. It is inhibited by coumarins and sulfonamide derivatives such as acetazolamide. At 6 weeks gestation, transcripts accumulate at low levels in the somites and at high levels throughout the notochord. As gestation continues, CA3 becomes abundant in all developing muscle masses and continues at high to moderate levels in the notochord.
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Conjugationhistidine
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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SourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolCA3-AS1, CA3, CPLX3, MGAT3, MGST3, P4HA3, CASP5, THAP4, IGHVIII-82, MT-CSB3
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Short nameRecombinant Carbonic Anhydrase III / CA3 Protein (His tag)
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
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LabelHis
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SpeciesHuman, Humans
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Alternative nameRec. H. sapiens Carbonic Anhydrase III / CA3 Protein (histidine detection labelled)
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Alternative techniquerec
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Gene info
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Identity
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Gene
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Long gene nameCA3 antisense RNA 1
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Locus
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Discovery year2015-04-24
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Entrez gene record
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RefSeq identity
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Classification
- Antisense RNAs
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecarbonic anhydrase 3
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Synonyms gene name
- carbonic anhydrase III, muscle specific
- carbonic anhydrase III
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Synonyms
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Carbonic anhydrases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecomplexin 3
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Synonyms
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GenBank acession
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Locus
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Discovery year2005-08-02
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namebeta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase
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Synonyms gene name
- mannosyl (beta-1,4-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase
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Synonyms
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GenBank acession
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Locus
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Discovery year1993-08-27
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Mannosyl-glycoprotein N-acetylglucosaminyltransferases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemicrosomal glutathione S-transferase 3
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1997-10-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Microsomal glutathione S-transferases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameprolyl 4-hydroxylase subunit alpha 3
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Synonyms gene name
- procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide III
- prolyl 4-hydroxylase, alpha polypeptide III
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2004-05-12
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecaspase 5
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Synonyms gene name
- caspase 5, apoptosis-related cysteine protease
- caspase 5, apoptosis-related cysteine peptidase
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Synonyms
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Locus
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Discovery year1996-09-13
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Caspases
- Caspase recruitment domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameTHAP domain containing 4
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2003-10-08
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- THAP domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable (III)-82 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable (III)-82
- immunoglobulin heavy variable (III)-82 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-17
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemitochondrially encoded conserved sequence block III
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Synonyms gene
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Synonyms gene name
- conserved sequence block III
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Synonyms
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Locus
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Discovery year1989-10-11
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Pubmed identfication
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Classification
- Mitochondrially encoded regions
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data