Recombinant Human CAMK1G / CLICK III / CaMKIgamma Protein (His & GST tag)
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Catalog numberPKSH030332-20µg
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PricePlease ask
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Size20µg
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SynonymCLICK3;CLICKIII;dJ272L16.1;RP1-272L16.2;VWS1
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ActivityNA
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SequenceMet 1-Met 476
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Fusion tagN-His & GST
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AccessionQ96NX5-1
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Expressed HostBaculovirus-Insect Cells
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ShippingKinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.Shipment made at ambient temperature may seriously affect the activity of the ordered products.
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Purity>85 % as determined by SDS-PAGE
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Endotoxin<1.0 EU per µg as determined by the LAL method
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Stability and StorageSamples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Mol Mass81 kDa
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AP Mol Mass75 kDa
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FormulationSupplied as sterile 50mM Tris, 100mM NaCl, pH 8.0, 20% gly, 0.3mM DTT
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BackgroundCalmodulin-Dependent Protein Kinase (CaM Kinase) is a kind of protein phosphorylate multiple downstream targets. Concentration of cytosolic calcium functions as a second messenger that mediates a wide range of cellular responses. Calcium binds to calcium binding proteins (calmodulin/CaM) and stimulates the activity of a variety of enzymes, including CaM kinases referred to as CaM-kinases (CaMKs), such as CaMKI, CaMKII, CaMKIV and CaMKK. Calmodulin-dependent protein kinase CL3/CaMKIγ is a memberane-anchored CaMK belonging to the CaM kinase family. Its C-terminal region is uniquely modified by two sequential lipidification steps: prenylation followed by a kinase-activity-regulated palmitoylation. These modifications are essential for CaMKIγ membrane anchoring and targeting into detergent-resistant lipid microdomains in the dendrites. It has been found that CaMKIγ critically contributed to BDNF-stimulated dendritic growth. Raft insertion of CaMKIγ specifically promoted dendritogenesis of cortical neurons by acting upstream of RacGEF STEF and Rac, both present in lipid rafts. Thus, CaMKIγ may represent a key element in the Ca2+-dependent and lipid-raft-delineated switch that turns on extrinsic activity-regulated dendrite formation in developing cortical neurons.
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Conjugationhistidine
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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SourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolMGST3, CAMK1G, CPLX3, MGAT3, P4HA3, CASP5, THAP4, IGHVIII-82, MT-CSB3, IGHVIII-67-4
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Short nameRecombinant CAMK1G / CLICK III / CaMKIgamma Protein (His & GST tag)
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
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LabelHis
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SpeciesHuman, Humans
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Alternative nameRec. H. sapiens calcium/calmodulin-dependent protein phosphorylation catalyst IG / CLICK III / CaMKIgamma Protein (histidine & GST detection labelled)
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Alternative techniquerec
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Alternative to gene targetcalcium/calmodulin-dependent protein kinase IG, CAMK1G and IDBG-106416 and ENSG00000008118 and 57172, transferase activity, Plasma membranes, Camk1g and IDBG-209251 and ENSMUSG00000016179 and 215303, BT.30982 and IDBG-638399 and ENSBTAG00000016541 and 511504
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Gene info
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Identity
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Gene
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Long gene namemicrosomal glutathione S-transferase 3
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1997-10-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Microsomal glutathione S-transferases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecalcium/calmodulin dependent protein kinase IG
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Synonyms gene name
- calcium/calmodulin-dependent protein kinase IG
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Synonyms
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Locus
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Discovery year2001-03-21
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Calmodulin dependent protein kinases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecomplexin 3
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Synonyms
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GenBank acession
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Locus
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Discovery year2005-08-02
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namebeta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase
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Synonyms gene name
- mannosyl (beta-1,4-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase
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Synonyms
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GenBank acession
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Locus
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Discovery year1993-08-27
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Mannosyl-glycoprotein N-acetylglucosaminyltransferases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameprolyl 4-hydroxylase subunit alpha 3
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Synonyms gene name
- procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide III
- prolyl 4-hydroxylase, alpha polypeptide III
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2004-05-12
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecaspase 5
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Synonyms gene name
- caspase 5, apoptosis-related cysteine protease
- caspase 5, apoptosis-related cysteine peptidase
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Synonyms
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Locus
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Discovery year1996-09-13
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Caspases
- Caspase recruitment domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameTHAP domain containing 4
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2003-10-08
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- THAP domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable (III)-82 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable (III)-82
- immunoglobulin heavy variable (III)-82 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-17
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemitochondrially encoded conserved sequence block III
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Synonyms gene
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Synonyms gene name
- conserved sequence block III
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Synonyms
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Locus
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Discovery year1989-10-11
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Pubmed identfication
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Classification
- Mitochondrially encoded regions
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable (III)-67-4 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable (III)-67-4
- immunoglobulin heavy variable (III)-67-4 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-17
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data