Recombinant Human Biotinidase / Biotinase / BTD Protein (His tag) 20µg

• Catalog number
  PKSH030529-20µg
• Price
  Please ask
• Size
  20µg

• Synonym
  BTD
• Activity
  Measured by its ability to hydrolyze biocytin to lysine and biotin. The specific activity is >500pmol/min/μg.
• Sequence
  Met1-Asp543
• Fusion tag
  C-His
• Accession
  P43251
• Expressed Host
  HEK293 Cells
• Shipping
  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
• Purity
  ＞95 % as determined by SDS-PAGE
• Endotoxin
  ＜1.0 EU per µg as determined by the LAL method
• Stability and Storage
  Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Mol Mass
  58.2 kDa
• AP Mol Mass
  66-76 kDa
• Formulation
  Lyophilized from sterile PBS, pH 7.4
• Reconstitution
  Please refer to it for detailed information.
• Background
  Biotinidase, also known as biotinase and BTD, is a ubiquitous mammalian cell enzyme which expressed at high levels in the liver, serum, and kidney. Its primary function is to cleave biotin from biocytin, preserving the pool of biotin for use as a cofactor for biotin dependent enzymes, namely the 4 human carboxylases. Biotinidase also recycles biotin from enzymes in the body that use it as a helper component in order to function. These enzymes, known ascarboxylases, are important in the processing of fats, carbohydrates, and proteins. Biotin is attached to these carboxylase enzymes through an amino acid (the building material of proteins) called lysine, forming a complex calledbiocytin.
• Product URL
• PDF URL
• Conjugation
  histidine
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Biotinidase   Biotinase   BTD   Protein   His   tag   20µg  
• Gene symbol
  BTD
• Short name
  Recombinant Biotinidase / Biotinase / BTD Protein (His tag) 20µg
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
• Label
  His
• Species
  Human, Humans
• Alternative name
  Rec. H. sapiens Biotinidase / Biotinase / BTD Protein (histidine detection labelled) 20µg
• Alternative technique
  rec

Gene info

• Identity
  HGNC:1122
• Gene
  BTD
• Long gene name
  biotinidase
• Synonyms name
  • biotinase
• GenBank acession
  • AF018631
• Locus
  3p25.1
• Discovery year
  1994-03-30
• Entrez gene record
  686
• Pubmed identfication
  • 8001986
• RefSeq identity
  • NM_000060
• Classification
  • Vanin family
• VEGA ID
  OTTHUMG00000129861

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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