Recombinant Human ALCAM / CD166 Protein (His tag) 200µg
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Catalog number
PKSH031919-200µg
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Price
Please ask
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Size
200µg
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Synonym
CD166;MEMD
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Activity
Immobilized human ALCAM-His at 10 μg/ml (100 μl/well) can bind mouse CD6-Fc , The EC50 of mouse CD6-Fc is 0.1-0.22 μg/ml.
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Sequence
Met 1-Ala 526
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Fusion tag
C-His
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Accession
NP_001618.2
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Expressed Host
HEK293 Cells
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Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
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Purity
＞98 % as determined by SDS-PAGE
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Endotoxin
＜1.0 EU per µg as determined by the LAL method
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Stability and Storage
Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Mol Mass
57.4 kDa
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AP Mol Mass
60-70 kDa
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Formulation
Lyophilized from sterile PBS, pH 7.4
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Reconstitution
Please refer to it for detailed information.
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Background
Activated leukocyte cell adhesion molecule (ALCAM)/Cluster of differentiation (CD166) is a type I transmembrane cell adhesion molecule belonging to the Ig superfamily and a ligand for CD6 that is expressed on T lymphocytes. The extracellular domain of ALCAM contains five Ig-like domains (three Ig-like C2-type domains and two Ig-like V-type domains), of which the amino-terminal V1 domain is essential for ligand binding and ALCAM-mediated cell aggregation. ALCAM mediates both heterophilic (ALCAM-CD6) and homophilic (ALCAM-ALCAM) cell-cell interactions. ALCAM/CD6 interaction plays a role in T cell development and T cell regulation, as well as in the binding of T- and B-cells to activated leukocytes. Recently, homophilic (ALCAM-ALCAM) adhesion was shown to play important roles in tight cell-to-cell interaction and regulation of stem cell differentiation. While expressed in a wide variety of tissues, ALCAM is usually restricted to subsets of cells involved in dynamic growth and/or migration, including neural development, branching organ development, hematopoiesis, immune response and tumor progression. And CD166 is regarded as a potential novel breast cancer indicator and therapeutic target.
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Conjugation
histidine
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
ALCAM
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Short name
Recombinant ALCAM / CD166 Protein (His tag) 200µg
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
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Label
His
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Species
Human, Humans
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Alternative name
Rec. H. sapiens activated leukocyte cellular adhesion molecule / CD166 Protein (histidine detection labelled) 200µg
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Alternative technique
rec
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Alternative to gene target
activated leukocyte cell adhesion molecule, CD166 and MEMD, ALCAM and IDBG-48383 and ENSG00000170017 and 214, protein binding, Cell surfaces, Alcam and IDBG-165765 and ENSMUSG00000022636 and 11658, ALCAM and IDBG-631637 and ENSBTAG00000000088 and 281614
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Gene info
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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