Rat sICAM-1/CD54(Soluble Intercellular Adhesion Molecule 1) EIA Kit
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Catalog number
E-EL-R0046-96T
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Price
Please ask
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Size
96Test
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Detection Type
Colormetric
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Target Species
Rat
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Target Synonym
BB2, P3.58
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Test principle
This EIA kit uses the Sandwich-EIA principle. The micro EIA plate provided in this kit has been pre-coated with an antibody specific to Rat sICAM-1/CD54. Standards or samples are added to the micro EIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat sICAM-1/CD54 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat sICAM-1/CD54, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat sICAM-1/CD54. You can calculate the concentration of Rat sICAM-1/CD54 in the samples by comparing the OD of the samples to the standard curve.
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Sensitivity
0.23 ng/mL
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Detection Range
0.39~25 ng/mL
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Target Name
sICAM-1/CD54
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UNIProt ID
Q00238
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Tested Sample Types
Serum, Plasma, Cell supernatant
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Product Name
Rat sICAM-1/CD54(Soluble Intercellular Adhesion Molecule 1) EIA Kit
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Type
Sandwich
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Description
cell adhesion molecules play a role in cell growth and activation and are often identified by WB or ELISA as in the sICAM-1/CD54(Soluble Intercellular Adhesion Molecule 1) EIA Kit. This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C. Whole adhesion and interacting molecules are present in lysates used as reference for ELISA quantification of these molecules and their subunits.
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Properties
MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay
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About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin name
Rattus norvegicus
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Gene target
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Gene symbol
ICAM1
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Short name
sICAM-1/CD54(Soluble Intercellular Adhesion Molecule 1) EIA Kit
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Technique
EIA, eia
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Host
Rat
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Species
Rat, Rats
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Alternative name
Rat sICAM-1/CD54(Soluble Intercellular Adhesion Molecule 1) EIA reagent
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Alternative technique
kits, elisas
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
MeSH Data
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Name
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Concept
Scope note:
Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
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Tree numbers
- E05.478.566.350
- E05.478.583.400
- E05.601.470.350
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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