Rat SCF (Stem Cell Factor) CLIA Kit
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Catalog number
E-CL-R0618-96T
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Price
Please ask
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Size
96Test
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Detection Type
Colormetric
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Target Species
Rat
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Target Synonym
KITLG, FPH2, KL-1, Kitl, MGF, SF, SHEP7
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Test principle
This CLIA kit uses the Sandwich- CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat SCF . Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat SCF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat SCF , biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat SCF . You can calculate the concentration of Rat SCF in the samples by comparing the RLU value of the samples to the standard curve.
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Sensitivity
4.69pg/mL
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Detection Range
7.81~500pg/mL
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Target Name
SCF
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UNIProt ID
NA
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Tested Sample Types
Serum, Plasma, Cell supernatant
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Product Name
Rat SCF (Stem Cell Factor) CLIA Kit
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Type
Sandwich
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Description
For cells, cell lines and tissues in culture till half confluency. Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
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About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin name
Rattus norvegicus
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Test
Stem cell factors and stem cell growth factors will produce stem cells or be part of a transdifferentiation process to produce other cells. A cell can transdifferentiate by going back to the naive stem cell stadium or directly into the other cell, helped by the stem cell and transdifferentiationf actors. Stem cell growth factors or stem cell factors are mostly used to produce iPSCs or induced pluripotent stem cells by Jamaka or Thomson factors by using for example 5 Lenti-III-CMV viruses, expressing the Yamanaka iPSC factor set (Oct4, Sox2, Nanog and Lin28) + GFP positive control. Trans differentiation will omit the stem cell stadium but stem cell factors sill play an important role in trans differentiation strategies.
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Gene target
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Gene symbol
KITLG
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Short name
SCF (Stem Factor) CLIA Kit
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Host
Rat
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Species
Rat, Rats
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Alternative name
Rat SCF (progenitor cellular Factor) CLIA reagent
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Alternative technique
kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Tissue
cell, stem
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Gene info
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Identity
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Gene
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Long gene name
KIT ligand
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Synonyms gene
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1991-06-04
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
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Locus Specific Databases
Product images
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