Rat RHOA(Ras Homolog Gene Family, Memmber A) EIA Kit

  • Catalog number
    E-EL-R2590-96T
  • Price
    Please ask
  • Size
    96Test
  • Detection Type
    Colormetric
  • Target Species
    Rat
  • Target Synonym
    NA
  • Test principle
    This EIA kit uses the Sandwich-EIA principle. The micro EIA plate provided in this kit has been pre-coated with an antibody specific to Rat RHOA. Standards or samples are added to the micro EIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat RHOA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat RHOA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat RHOA. You can calculate the concentration of Rat RHOA in the samples by comparing the OD of the samples to the standard curve.
  • Sensitivity
    0.1ng/mL
  • Detection Range
    0.16~10ng/mL
  • Target Name
    RHOA
  • UNIProt ID
    NA
  • Tested Sample Types
    Serum, Plasma, Cell supernatant
  • Product Name
    Rat RHOA(Ras Homolog Gene Family, Memmber A) EIA Kit
  • Type
    Sandwich
  • Description
    This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C. cDNA genes are a locus (or region) of DNA for functional transcript RNA or protein. An ELISA is used to detect the expressed protein in biological fluids, serum, saliva.
  • Properties
    MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay
  • About
    Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
  • Latin name
    Rattus norvegicus
  • Gene target
    RHOA   Ras   Homolog   Gene   Family   Memmber   Kit  
  • Gene symbol
    RHOA-IT1, RHOA
  • Short name
    RHOA(Ras Homolog Family, Memmber A) EIA Kit
  • Technique
    EIA, eia
  • Host
    Rat
  • Species
    Rat, Rats
  • Alternative name
    Rat ras homolog family member A(Ras Homolog Gene family, Memmber A) EIA reagent
  • Alternative technique
    kits, elisas
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
Gene info
  • Identity
  • Gene
  • Long gene name
    RHOA intronic transcript 1
  • Synonyms gene name
    • RHOA intronic transcript 1 (non-protein coding)
  • Locus
  • Discovery year
    2011-05-24
  • Classification
    • Intronic transcripts
  • VEGA ID
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
  • Tree numbers
    • E05.478.566.350
    • E05.478.583.400
    • E05.601.470.350
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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