Rat PTPRO(Protein Tyrosine Phosphatase Receptor Type O) EIA Kit
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Catalog numberE-EL-R0825-96T
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Price:
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Size96Test
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Detection TypeColormetric
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Target SpeciesRat
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Target SynonymNA
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Test principleThis EIA kit uses the Sandwich-EIA principle. The micro EIA plate provided in this kit has been pre-coated with an antibody specific to Rat PTPRO. Standards or samples are added to the micro EIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PTPRO and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PTPRO, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat PTPRO. You can calculate the concentration of Rat PTPRO in the samples by comparing the OD of the samples to the standard curve.
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Sensitivity0.94ng/mL
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Detection Range1.56~100ng/mL
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Target NamePTPRO
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UNIProt IDNA
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Tested Sample TypesSerum, Plasma, Cell supernatant
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Product NameRat PTPRO(Protein Tyrosine Phosphatase Receptor Type O) EIA Kit
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TypeSandwich
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DescriptionThis ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C. The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
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PropertiesMeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay
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AboutRats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin nameRattus norvegicus
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Gene target
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Gene symbolPTPRU, PTPRO
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Short namePTPRO(Protein Tyrosine Phosphatase Receptor O) EIA Kit
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TechniqueEIA, eia
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HostRat
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SpeciesRat, Rats
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Alternative nameRat PTPRO(Protein Tyrosine Phosphatase Receptor classification O) EIA reagent
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Alternative techniquekits, elisas
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Alternative to gene targetv-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
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Identity
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Gene
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Long gene nameprotein tyrosine phosphatase receptor type U
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1999-10-28
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Entrez gene record
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Pubmed identfication
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Classification
- Protein tyrosine phosphatases receptor type
- Fibronectin type III domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameprotein tyrosine phosphatase receptor type O
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1995-02-22
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Entrez gene record
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Pubmed identfication
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Classification
- Protein tyrosine phosphatases receptor type
- Fibronectin type III domain containing
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
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Tree numbers
- E05.478.566.350
- E05.478.583.400
- E05.601.470.350
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data